Electron tomography of biological samples

被引：25

作者：

Marco, S

Boudier, T

Messaoudi, C

Rigaud, JL

机构：

[1] Inst Curie, CNRS, Sect Rech, UMR 168, F-75005 Paris, France

[2] CEA, LRC 34V, F-75005 Paris, France

来源：

BIOCHEMISTRY-MOSCOW | 2004年 / 69卷 / 11期

关键词：

electron microscopy; electron tomography; image analysis; 3D reconstruction;

D O I：

10.1007/s10541-005-0067-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electron tomography allows computing three-dimensional (3D) reconstructions of objects from their projections recorded at several angles. Combined with transmission electron microscopy, electron tomography has contributed greatly to the understanding of subcellular structures and organelles. Performed on frozen-hydrated samples, electron tomography has yielded useful information about complex biological structures. Combined with energy filtered transmission electron microscopy (EFTEM) it can be used to analyze the spatial distribution of chemical elements in biological or material sciences samples. In the present review, we present an overview of the requirements, applications, and perspectives of electron tomography in structural biology.

引用

页码：1219 / +

页数：8

共 66 条

[31] PATTERN-RECOGNITION AND CLASSIFICATION OF IMAGES OF BIOLOGICAL MACROMOLECULES USING ARTIFICIAL NEURAL NETWORKS [J].

MARABINI, R ;

CARAZO, JM .

BIOPHYSICAL JOURNAL, 1994, 66 (06) :1804-1814

[32] A variant to the ''random approximation'' of the reference-free alignment algorithm [J].

Marco, S ;

Chagoyen, M ;

delaFraga, LG ;

Carazo, JM ;

Carrascosa, JL .

ULTRAMICROSCOPY, 1996, 66 (1-2) :5-10

[33] Cryo-transmission electron Microscopy of frozen-hydrated sections of Escherichia coli and Pseudomonas aeruginosa [J].

Matias, VRF ;

Al-Amoudi, A ;

Dubochet, J ;

Beveridge, TJ .

JOURNAL OF BACTERIOLOGY, 2003, 185 (20) :6112-6118

[34] The emergence of electron tomography as an important tool for investigating cellular ultrastructure [J].

McEwen, BF ;

Marko, M .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2001, 49 (05) :553-563

[35] Electron tomographic and other approaches for imaging molecular machines [J].

McEwen, BF ;

Frank, J .

CURRENT OPINION IN NEUROBIOLOGY, 2001, 11 (05) :594-600

[36] Use of frozen-hydrated axonemes to assess imaging parameters and resolution limits in cryoelectron tomography [J].

McEwen, BF ;

Marko, M ;

Hsieh, CE ;

Mannella, C .

JOURNAL OF STRUCTURAL BIOLOGY, 2002, 138 (1-2) :47-57

[37] Cryoelectron microscopy and cryoelectron tomography of the nuclear pre-mRNA processing machine [J].

Medalia, O ;

Typke, D ;

Hegerl, R ;

Angenitzki, M ;

Sperling, J ;

Sperling, R .

JOURNAL OF STRUCTURAL BIOLOGY, 2002, 138 (1-2) :74-84

[38] Use of cryo-negative staining in tomographic reconstruction of biological objects: application to T4 bacteriophage [J].

Messaoudi, C ;

Boudier, T ;

Lechaire, JP ;

Rigaud, JL ;

Delacroix, H ;

Gaill, F ;

Marco, S .

BIOLOGY OF THE CELL, 2003, 95 (06) :393-398

[39] 3D electron microscopy in the physical sciences: the development of Z-contrast and EFTEM tomography [J].

Midgley, PA ;

Weyland, M .

ULTRAMICROSCOPY, 2003, 96 (3-4) :413-431

[40] Spectroscopic electron tomography [J].

Möbus, G ;

Doole, RC ;

Inkson, BJ .

ULTRAMICROSCOPY, 2003, 96 (3-4) :433-451

← 1 2 3 4 5 6 7 →