SEK1/MKK4, c-Jun and NFKB are differentially activated in forebrain neurons during postnatal development and injury in both control and p75NGFR-deficient mice

The common neurotrophin receptor (p75(NGFR))can signal in vitro through activation of the c-Jun N-terminal kinase (JNK) pathway and nuclear translocation of NFKB. Activation of JNK and its substrate c-Jun can lead to apoptosis. We investigated these activities in vivo by comparing immunoreactivity for phosphorylated(p) SEK-1 (or MKK4, which activates JNK), c-Jun (ser63, ser73) and nuclear translocation of NFKB-p50 in tissue sections through the forebrain of control and p75(NGFR)-deficient mice. During postnatal development, SEK1 p-immunoreactivity was detectable in p75(NGFR)-positive cholinergic neurons and p75(NGFR)-negative neurons throughout the forebrain in control mice, During development, few cells contained c-Junp, although many neurons contained c-Jun. No obvious c-Jun immunostaining was present in the adult forebrain. At any age, NFKB-p50 immunoreactivity was seen in nuclei of most cells throughout the forebrain. Following fimbria fornix transection in adult mice, few basal forebrain neurons contained SEK1p while many axotomized choline acetyltransferase (ChAT)-positive neurons contained c-Junp and nuclear NFKB-p50. The immunostaining patterns of SEK1p, c-Junp and NFKB during development and following injury were largely similar in p75(NGFR) deficient mice. During development, cells throughout the forebrain had TdT-mediated dUTP-biotin nick end labelling (TUNEL)-labelling (a potential marker for apoptosis), however, their presence was not predicted by number of neurons stained for SEK1 p or c-Junp. These results suggest that the expected activation of the JNK pathway by p75(NGFR), as well as the expected relationship between SEK1 and downstream activation of c-Jun do not occur in the mammalian forebrain. Also, these results suggest that this activation does not necessarily lead to cell death.