High-Efficient FLPo Deleter Mice in C57BL/6J Background

被引：60

作者：

Wu, Yingjie ^{[1
]}

Wang, Chunxin ^{[2
]}

Sun, Hui ^{[1
]}

LeRoith, Derek ^{[1
]}

Yakar, Shoshana ^{[1
]}

机构：

[1] Mt Sinai Sch Med, Endocrinol Diabet & Bone Dis Div, New York, NY USA

[2] NINDS, Biochem Sect, SNB, NIH, Bethesda, MD 20892 USA

来源：

PLOS ONE | 2009年 / 4卷 / 11期

关键词：

TRANSGENIC MICE; CRE RECOMBINASE; MAMMALIAN-CELLS; EXPRESSION; GENE; WIDESPREAD; MOUSE;

D O I：

10.1371/journal.pone.0008054

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Conditional gene manipulation in mice becomes a routine for genetic studies of mammalian gene functions. Additional site-specific recombinases such as FLP or phi 31 provide one more level of gene manipulation flexibility. The recombination activity of the currently available FLP deleter mice remains low. We generated a new FLP deleter mouse line with the mouse codon-optimized FLPo gene in C57BJ/6 background, which showed superior recombination efficacy in comparison to FLPe deleter mice. 100% complete removal of FRT-flanked Neo cassette was observed in all F1 progeny mice carrying both FLPo and Neo cassette, which can be transmitted to F2 generation independent of FLPo activity. Our new FLPo transgenic mice (on pure C57BJ/6 background) will largely facilitate the gene targeting process and is valuable for conditional gene manipulation.

引用

页数：6

共 20 条

[1] Enhanced efficiency through nuclear localization signal fusion on phage φC31-integrase:: activity comparison with Cre and FLPe recombinase in mammalian cells [J].

Andreas, S ;

Schwenk, F ;

Küter-Luks, B ;

Faust, N ;

Kühn, R .

NUCLEIC ACIDS RESEARCH, 2002, 30 (11) :2299-2306

[2] Improved properties of FLP recombinase evolved by cycling mutagenesis [J].

Buchholz, F ;

Angrand, PO ;

Stewart, AF .

NATURE BIOTECHNOLOGY, 1998, 16 (07) :657-662

[3] Flp recombinase promotes site-specific DNA recombination in embryonic stem cells and transgenic mice [J].

Dymecki, SM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (12) :6191-6196

[4]

Farley FW, 2000, GENESIS, V28, P106, DOI 10.1002/1526-968X(200011/12)28:3/4<106::AID-GENE30>3.0.CO

[5]

2-T

[6] TARGETED DELETION OF 5'HS2 OF THE MURINE BETA-GLOBIN LCR REVEALS THAT IT IS NOT ESSENTIAL FOR PROPER REGULATION OF THE BETA-GLOBIN LOCUS [J].

FIERING, S ;

EPNER, E ;

ROBINSON, K ;

ZHUANG, Y ;

TELLING, A ;

HU, M ;

MARTIN, DIK ;

ENVER, T ;

LEY, TT ;

GROUDINE, M .

GENES & DEVELOPMENT, 1995, 9 (18) :2203-2213

[7] High-efficiency CAG-FLPe deleter mice in C57BL/6J background [J].

Kanki, H ;

Suzuki, H ;

Itohara, S .

EXPERIMENTAL ANIMALS, 2006, 55 (02) :137-141

[8] Activities of Various FLP Recombinases Expressed by Adenovirus Vectors in Mammalian Cells [J].

Kondo, Saki ;

Takata, Yuki ;

Nakano, Masakazu ;

Saito, Izumu ;

Kanegae, Yumi .

JOURNAL OF MOLECULAR BIOLOGY, 2009, 390 (02) :221-230

[9] MURINE PGK-1 PROMOTER DRIVES WIDESPREAD BUT NOT UNIFORM EXPRESSION IN TRANSGENIC MICE [J].

MCBURNEY, MW ;

STAINES, WA ;

BOEKELHEIDE, K ;

PARRY, D ;

JARDINE, K ;

PICKAVANCE, L .

DEVELOPMENTAL DYNAMICS, 1994, 200 (04) :278-293

[10] An Fgf8 mutant allelic series generated by Cre- and Flp-mediated recombination [J].

Meyers, EN ;

Lewandoski, M ;

Martin, GR .

NATURE GENETICS, 1998, 18 (02) :136-141

← 1 2 →