A genomic integration method to visualize localization of endogenous mRNAs in living yeast

被引：86

作者：

Haim, Liora ^{[1
]}

Zipor, Gadi ^{[1
]}

Aronov, Stella ^{[1
]}

Gerst, Jeffrey E. ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

来源：

NATURE METHODS | 2007年 / 4卷 / 05期

关键词：

D O I：

10.1038/NMETH1040

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

mRNA localization may be an important determinant for protein localization. We describe a simple PCR-based genomic-tagging strategy (m-TAG) that uses homologous recombination to insert binding sites for the RNA-binding MS2 coat protein (MS2-CP) between the coding region and 3' untranslated region (UTR) of any yeast gene. Upon coexpression of MS2-CP fused with GFP, we demonstrate the localization of endogenous mRNAs (ASH1, SRO7, PEX3 and OXA1) in living yeast (Saccharomyces cerevisiae).

引用

页码：409 / 412

页数：4

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