A fast and inexpensive DNA extraction/purification protocol for brown macroalgae

被引：47

作者：

Hoarau, Galice ^{[1
]}

Coyer, James A. ^{[1
]}

Stam, Wytze T. ^{[1
]}

Olsen, Jeanine L. ^{[1
]}

机构：

[1] Univ Groningen, Ctr Ecol & Evolutionary Studies, Dept Marine Benth Ecol & Evolut, NL-9750 AA Haren, Netherlands

来源：

MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 02期

关键词：

brown algae; DNA extraction; heterokonts; high throughput; Phaeophyceae;

D O I：

10.1111/j.1471-8286.2006.01587.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we describe a rapid method for extracting DNA from dried brown algae material using a microtitre plate system in conjunction with a milling instrument. The method allows the preparation of nuclear and organelle DNA of quality suitable for polymerase chain reaction amplification. It combines high throughput with low cost per sample: DNA from 192 samples can be extracted in c. 3 h for < EURO0.40 per sample, nearly tenfold cheaper than commercially available kits. Furthermore, by using microtitre plates, efficient storage and downstream processing is facilitated.

引用

收藏

页码：191 / 193

页数：3

相关论文

共 14 条

[1] Genetic isolation between three closely related taxa:: Fucus vesiculosus, F-Spiralis, and F-Ceranoides (Phaophyceae) [J].

Billard, E ;

Daguin, C ;

Pearson, G ;

Serrao, E ;

Engel, C ;

Valero, M .

JOURNAL OF PHYCOLOGY, 2005, 41 (04) :900-905

[2] Isolation and characterization of microsatellite markers in the nuclear genome of the brown alga Laminaria digitata (Phaeophyceae) [J].

Billot, C ;

Rousvoal, S ;

Estoup, A ;

Epplen, JT ;

Saumitou-Laprade, P ;

Valero, M ;

Kloareg, B .

MOLECULAR ECOLOGY, 1998, 7 (12) :1778-1780

[3]

BILLOT C, 1999, THESIS I NATL AGRONO

[4] Molecular identification of two sibling species under the name Gracilaria chilensis (Rhodophyta, Gracilariales) [J].

Cohen, S ;

Faugeron, S ;

Martínez, EA ;

Correa, JA ;

Viard, F ;

Destombe, C ;

Valer, M .

JOURNAL OF PHYCOLOGY, 2004, 40 (04) :742-747

[5] Evolution of Macrocystis spp. (Phaeophyceae) as determined by ITS1 and ITS2 sequences [J].

Coyer, JA ;

Smith, GJ ;

Andersen, RA .

JOURNAL OF PHYCOLOGY, 2001, 37 (04) :574-585

[6] A mtDNA-based phylogeny of the brown algal genus Fucus (Heterokontophyta; Phaeophyta) [J].

Coyer, JA ;

Hoarau, G ;

Oudot-Le Secq, MP ;

Stam, WT ;

Olsen, JL .

MOLECULAR PHYLOGENETICS AND EVOLUTION, 2006, 39 (01) :209-222

[7] GENETIC-VARIABILITY WITHIN A POPULATION AND BETWEEN DIPLOID/HAPLOID TISSUE OF MACROCYSTIS-PYRIFERA (PHAEOPHYCEAE) [J].

COYER, JA ;

ROBERTSON, DL ;

ALBERTE, RS .

JOURNAL OF PHYCOLOGY, 1994, 30 (03) :545-552

[8] Inheritance patterns of ITS1, chloroplasts and mitochondria in artificial hybrids of the seaweeds Fucus serratus and F-evanescens (Phaeophyceae) [J].

Coyer, JA ;

Peters, AF ;

Hoarau, G ;

Stam, WT ;

Olsen, JL .

EUROPEAN JOURNAL OF PHYCOLOGY, 2002, 37 (02) :173-178

[9] Characterization of microsatellite loci in the marine seaweeds, Fucus serratus and F-evanescens (Heterokontophyta; Fucaceae) [J].

Coyer, JA ;

Veldsink, JH ;

Jones, K ;

Stam, WT ;

Olsen, JL .

MOLECULAR ECOLOGY NOTES, 2002, 2 (01) :35-37

[10] An inexpensive and high-throughput procedure to extract and purify total genomic DNA for population studies [J].

Elphinstone, MS ;

Hinten, GN ;

Anderson, MJ ;

Nock, CJ .

MOLECULAR ECOLOGY NOTES, 2003, 3 (02) :317-320