Appearance of a stress-response protein, phage-shock protein A, in Escherichia coli exposed to hydrophobic organic solvents

被引:65
作者
Kobayashi, H [1 ]
Yamamoto, M [1 ]
Aono, R [1 ]
机构
[1] Tokyo Inst Technol, Fac Biosci & Biotechnol, Dept Bioengn, Midori Ku, Yokohama, Kanagawa 226, Japan
来源
MICROBIOLOGY-UK | 1998年 / 144卷
关键词
Escherichia coli; organic solvent; phage shock protein A; psp operon;
D O I
10.1099/00221287-144-2-353
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A 28 kDa protein associated with the inner membrane was induced strongly in Escherichia coli K-12 cells grown in the presence of a hydrophobic organic solvent, n-hexane or cyclooctane. These organic solvents suppressed the growth (growth rate and yield) of E. coli. A partial amino acid sequence showed that this protein was the phage-shock protein PspA. PspA is known to be induced in E. coli cells under extreme stress conditions. The results suggest that E. coli cells are subject to strong stress in the presence of organic solvents. Introduction of a multi-copy plasmid vector carrying the psp operon into E. coli improved the survival frequency of cells exposed suddenly to n-hexane but not the growth rate of cells growing in the presence of n-hexane.
引用
收藏
页码:353 / 359
页数:7
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