Appearance of a stress-response protein, phage-shock protein A, in Escherichia coli exposed to hydrophobic organic solvents

被引:65
作者
Kobayashi, H [1 ]
Yamamoto, M [1 ]
Aono, R [1 ]
机构
[1] Tokyo Inst Technol, Fac Biosci & Biotechnol, Dept Bioengn, Midori Ku, Yokohama, Kanagawa 226, Japan
来源
MICROBIOLOGY-UK | 1998年 / 144卷
关键词
Escherichia coli; organic solvent; phage shock protein A; psp operon;
D O I
10.1099/00221287-144-2-353
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A 28 kDa protein associated with the inner membrane was induced strongly in Escherichia coli K-12 cells grown in the presence of a hydrophobic organic solvent, n-hexane or cyclooctane. These organic solvents suppressed the growth (growth rate and yield) of E. coli. A partial amino acid sequence showed that this protein was the phage-shock protein PspA. PspA is known to be induced in E. coli cells under extreme stress conditions. The results suggest that E. coli cells are subject to strong stress in the presence of organic solvents. Introduction of a multi-copy plasmid vector carrying the psp operon into E. coli improved the survival frequency of cells exposed suddenly to n-hexane but not the growth rate of cells growing in the presence of n-hexane.
引用
收藏
页码:353 / 359
页数:7
相关论文
共 32 条
[11]  
DESMET MJ, 1983, ENZYME MICROB TECHNO, V5, P352
[12]   Biodegradation of indole at high concentration by persolvent fermentation with Pseudomonas sp. ST-200 [J].
Doukyu, N ;
Aono, R .
EXTREMOPHILES, 1997, 1 (02) :100-105
[13]   BIOCONVERSION OF N-OCTANE TO OCTANOIC-ACID BY A RECOMBINANT ESCHERICHIA-COLI CULTURED IN A 2-LIQUID PHASE BIOREACTOR [J].
FAVREBULLE, O ;
SCHOUTEN, T ;
KINGMA, J ;
WITHOLT, B .
BIO-TECHNOLOGY, 1991, 9 (04) :367-371
[14]   2-LIQUID PHASE BIOCATALYSIS - EPOXIDATION OF 1,7-OCTADIENE BY PSEUDOMONAS-PUTIDA [J].
HARBRON, S ;
SMITH, BW ;
LILLY, MD .
ENZYME AND MICROBIAL TECHNOLOGY, 1986, 8 (02) :85-88
[15]   A PSEUDOMONAS THRIVES IN HIGH-CONCENTRATIONS OF TOLUENE [J].
INOUE, A ;
HORIKOSHI, K .
NATURE, 1989, 338 (6212) :264-266
[16]   REPLICATION IN SACCHAROMYCES-CEREVISIAE OF PLASMID PBR313 CARRYING DNA FROM THE YEAST TRPL REGION [J].
KINGSMAN, AJ ;
CLARKE, L ;
MORTIMER, RK ;
CARBON, J .
GENE, 1979, 7 (02) :141-152
[17]   Involvement of stress protein PspA (phage shock protein A) of Escherichia coli in maintenance of the protonmotive force under stress conditions [J].
Kleerebezem, M ;
Crielaard, W ;
Tommassen, J .
EMBO JOURNAL, 1996, 15 (01) :162-171
[18]  
KLEEREBEZEM M, 1993, MOL MICROBIOL, V7, P957
[19]   THE PHYSICAL MAP OF THE WHOLE ESCHERICHIA-COLI CHROMOSOME - APPLICATION OF A NEW STRATEGY FOR RAPID ANALYSIS AND SORTING OF A LARGE GENOMIC LIBRARY [J].
KOHARA, Y ;
AKIYAMA, K ;
ISONO, K .
CELL, 1987, 50 (03) :495-508
[20]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+