Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12

被引：58

作者：

Kato, A ^{[1
]}

Ohnishi, H ^{[1
]}

Yamamoto, K ^{[1
]}

Furuta, E ^{[1
]}

Tanabe, H ^{[1
]}

Utsumi, R ^{[1
]}

机构：

[1] Kinki Univ, Dept Agr Chem, Nara 6318505, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2000年 / 64卷 / 06期

关键词：

drug efflux; two-component system; EvgA-EvgS; EmrK-EmrY;

D O I：

10.1271/bbb.64.1203

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spontaneous mutations have been isolated in Escherichia coli that result in the constitutive expression of an emrKY promoter. These mutations were found to be single-nucleotide substitutions within the linker region of the sensor protein EvgS, which is part of a two-component regulatory system along with EvgA. in the linker mutants (evgS1 and evgS4), emrKY expression became constitutive and MIC against sodium deoxycholate was 20 mg/ml, eight-fold higher than in the wild type. Furthermore, the start site of transcription from the promoter of emrKY was identified; EvgA was shown to bind at the -52 to -84 region by the footprinting experiment.

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收藏

页码：1203 / 1209

页数：7

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