Biochemical and phylogenetic characterization of the dUTPase from the archaeal virus SIRV

被引：34

作者：

Prangishvili, D ^{[1
]}

Klenk, HP ^{[1
]}

Jakobs, G ^{[1
]}

Schmiechen, A ^{[1
]}

Hanselmann, C ^{[1
]}

Holz, I ^{[1
]}

Zillig, W ^{[1
]}

机构：

[1] Max Planck Inst Biochem, D-82152 Martinsried, Germany

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1998年 / 273卷 / 11期

关键词：

D O I：

10.1074/jbc.273.11.6024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The derived amino acid sequence from a 474-base pair open reading frame in the genome of the Sulfolobus islandicus rod-shaped virus SIRV shows striking similarity to bacterial dCTP deaminases and to dUTPases from eukaryotes, bacteria, Poxviridae, and Retroviridae, The putative gene was expressed in Escherichia coli, and dUTPase activity of the recombinant enzyme was demonstrated by hydrolysis of dUTP to dUMP. Deamination of dCTP by the enzyme was not detected, Phylogenetic analysis based on amino acid sequences of the characterized enzyme and its homologues showed that the dUTPase-encoding dut genes and the dCTP deaminase encoding dcd genes constitute a paralogous gene family, This report is the first identification and functional characterization of an archaeal dUTPase and the first phylogeny derived for the dcd-dut gene family.

引用

页码：6024 / 6029

页数：6

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