Three-dimensional chitin-based scaffolds from Verongida sponges (Demospongiae: Porifera). Part I. Isolation and identification of chitin

被引：108

作者：

Ehrlich, H. ^{[1
]}

Ilan, M. ^{[2
]}

Maldonado, M. ^{[3
]}

Muricy, G. ^{[4
]}

Bavestrello, G. ^{[5
]}

Kljajic, Z. ^{[6
]}

Carballo, J. L. ^{[7
]}

Schiaparelli, S. ^{[8
]}

Ereskovsky, A. ^{[9
]}

Schupp, P. ^{[10
]}

Born, R. ^{[11
,12
]}

Worch, H. ^{[11
,12
]}

Bazhenov, V. V. ^{[13
]}

Kurek, D. ^{[14
]}

Varlamov, V. ^{[14
]}

Vyalikh, D. ^{[15
]}

Kummer, K. ^{[15
]}

Sivkov, V. V. ^{[16
]}

Molodtsov, S. L. ^{[15
]}

Meissner, H. ^{[17
]}

Richter, G. ^{[17
]}

Steck, E. ^{[18
]}

Richter, W. ^{[18
]}

Hunoldt, S. ^{[1
]}

Kammer, M. ^{[1
]}

Paasch, S. ^{[1
]}

Krasokhin, V. ^{[19
]}

Patzke, G. ^{[20
]}

Brunner, E. ^{[1
]}

机构：

[1] Tech Univ Dresden, Inst Bioanalyt Chem, D-01069 Dresden, Germany

[2] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Zool, IL-69978 Tel Aviv, Israel

[3] Ctr Estudios Avanzados Blanes, Dept Marine Ecol, Blanes 17300, Girona, Spain

[4] Univ Fed Rio de Janeiro, Museu Nacl, Dept Invertebrados, BR-20940040 Rio De Janeiro, Brazil

[5] Univ Politecn Marche, Dipartimento Sci Mare, I-60131 Ancona, Italy

[6] Univ Montenegro, Inst Marine Biol, Kotor 85330, Montenegro

[7] Univ Nacl Autonoma Mexico, Estn Mazatlan, Inst Ciencias Mar & Limnol, Mazatlan 82040, Sin, Mexico

[8] Nat Hist Museum, I-16100 Genoa, Italy

[9] Aix Marseille Univ, CNRS, DIMAR, Ctr Oceanol Marseille,Stn Marine Endoume,UMR 6540, F-13007 Marseille, France

[10] UOG Stn, UOG Marine Lab, Mangilao, GU 96923 USA

[11] Tech Univ Dresden, Max Bergmann Ctr Biomat, D-01069 Dresden, Germany

[12] Tech Univ Dresden, Inst Mat Sci, D-01069 Dresden, Germany

[13] Far Eastern Natl Univ, Inst Chem & Appl Ecol, Vladivostok 690650, Russia

[14] Russian Acad Sci, Ctr Bioengn, Moscow 117312, Russia

[15] Tech Univ Dresden, Inst Solid State Phys, D-01069 Dresden, Germany

[16] Russian Acad Sci, Komi Sc UrD, Dept Math, Syktyvkar 167000, Russia

[17] Tech Univ Dresden, Carl Gustav Carus Univ Clin, D-01069 Dresden, Germany

[18] Heidelberg Univ, Orthoped Clin, D-69118 Heidelberg, Germany

[19] Russian Acad Sci, Pacific Inst Bioorgan Chem, Far Eastern Branch, Vladivostok 690022, Russia

[20] Univ Zurich, Inst Inorgan Chem, CH-8057 Zurich, Switzerland

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2010年 / 47卷 / 02期

关键词：

Chitin; Scaffold; Sponges; BROMINATED COMPOUNDS; DEMOSPONGIAE; SKELETONS; AEROPHOBA; CUTICLE; SOLIDS; FIBERS; NMR;

D O I：

10.1016/j.ijbiomac.2010.05.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Marine invertebrate organisms including sponges (Porifera) not only provide an abundant source of biologically active secondary metabolites but also inspire investigations to develop biomimetic composites, scaffolds and templates for practical use in materials science, biomedicine and tissue engineering. Here, we presented a detailed study of the structural and physico-chemical properties of three-dimensional skeletal scaffolds of the marine sponges Aiolochroia crassa, Aplysina aerophoba, A. cauliformis. A. cavernicola, and A. fulva (Verongida: Demospongiae). We show that these fibrous scaffolds have a multilayered design and are made of chitin. C-13 solid-state NMR spectroscopy, NEXAFS, and IR spectroscopy as well as chitinase digestion and test were applied in order to unequivocally prove the existence of alpha-chitin in all investigated species. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：132 / 140

页数：9

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