Functional nicotinic ACh receptors on interneurones in the rat hippocampus

被引:234
作者
Jones, S [1 ]
Yakel, JL [1 ]
机构
[1] NIEHS,LAB SIGNAL TRANSDUCT,NIH,RES TRIANGLE PK,NC 27709
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1997年 / 504卷 / 03期
关键词
D O I
10.1111/j.1469-7793.1997.603bd.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. Neuronal nicotinic ACh receptors (nAChRs) were studied in the rat hippocampal slice preparation using whole-cell patch-clamp recording techniques. 2. Responses to ACh (100 mu M) were detected on inhibitory interneurones in the CA1 field of the hippocampus proper and in the dentate gyrus, but not on principal excitatory neurones in either region. The different neuronal types were identified based on their morphology and location. 3. ACh excited interneurones in the hippocampus and dentate gyrus in current-clamp recordings. In voltage-clamp recordings, ACh-activated inward currents were recorded from interneurones in the presence of blockers of synaptic transmission and the muscarinic ACh receptor antagonist atropine. The zero current potential for this response to ACh was near 0 mV. 4. The effect of ACh was mimicked by the nAChR-selective agonists nicotine (100 mu M) and 1,1-dimethyl-4-phenyl-piperazinium iodide (DMPP, 100 mu M). The response to ACh was reversibly antagonized by the neuronal nAChR antagonist mecamylamine (10 mu M). The nAChR alpha 7 subunit-selective antagonists alpha-bungarotoxin (100 nM) and methyllycaconitine (10 nM) also inhibited the response to ACh. 5. These observations demonstrate the presence of functional nAChRs on inhibitory interneurones in the rat hippocampus. Thus, a novel mechanism by which ACh can regulate neuronal activity in the hippocampus is revealed.
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页码:603 / 610
页数:8
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