An Electrochemical DNA Biosensor for the Detection of the Apa I Polymorphism in the Vitamin D Receptor Gene Using Meldola's Blue as a Hybridization Indicator

被引：9

作者：

Aladag, Nilay ^{[1
]}

Ozkan-Ariksoysal, Dilsat ^{[1
]}

Gezen-Ak, Duygu ^{[2
]}

Yilmazer, Selma ^{[2
]}

Ozsoz, Mehmet ^{[1
]}

机构：

[1] Ege Univ, Dept Analyt Chem, Fac Pharm, TR-35100 Izmir, Turkey

[2] Istanbul Univ, Dept Med Biol, Cerrahpasa Fac Med, Istanbul, Turkey

来源：

ELECTROANALYSIS | 2010年 / 22卷 / 05期

关键词：

Electrochemical DNA biosensor; Meldola's blue; Intercalator; Vitamin D receptor; Apa I polymorphism; Biosensors; Polymorphism; HEPATITIS-B-VIRUS; METHYLENE-BLUE; NUCLEOSIDE TRIPHOSPHATES; IMPEDANCE SPECTROSCOPY; MODIFIED ELECTRODE; MODIFIED SURFACES; GUANINE SIGNAL; NUCLEIC-ACIDS; START CODON; GENOSENSOR;

D O I：

10.1002/elan.200900405

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Electrochemical detection of nucleic acid base mismatches related to Apa I single nucleotide polymorphism (SNP) in the vitamin D receptor gene was performed successfully using 7-dimethyl-amino-1,2-benzophenoxazinium salt (Meldola's blue, MDB) with 10.9 pmo1/100 mu L of detection limit. MDB reduction signals obtained from probe, mismatch(probe-SNP containing target) and hybrid(probe-target) modified pencil graphite electrode(PGE) increased respectively. The sensor was able to clearly distinguish perfect match from mismatch DNA in a 30 min. detection time. Several factors affecting on the hybridization and indicator response are studied to maximize sensitivity and selectivity. The advantages of the biosensor are discussed in comparison with previous electrochemical assays for DNA hybridization.

引用

页码：590 / 598

页数：9

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