Aminophenyl- and nitrophenyl-labeled nucleoside triphosphates:: Synthesis, enzymatic incorporation, and electrochemical detection

被引：144

作者：

Cahova, Hana ^{[2
]}

Havran, Ludek ^{[1
]}

Brazdilova, Petra ^{[1
]}

Pivonkova, Hana ^{[1
]}

Pohl, Radek ^{[2
]}

Fojta, Miroslav ^{[1
]}

Hocek, Michal ^{[2
]}

机构：

[1] Acad Sci Czech Republ, Inst Biophys, CS-61265 Brno, Czech Republic

[2] Acad Sci Czech Republ, Inst Organ Chem & Biochem, Gilead Sci & IOCB Res Ctr, CR-16610 Prague 6, Czech Republic

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 11期

关键词：

DNA; electrochemistry; nucleobases; nucleosides; oligonucleotides;

D O I：

10.1002/anie.200705088

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Primer extension is used to incorporate labeled nucleoside triphosphates into oligonucleotides (ONs). Aminophenyl and nitrophenyl modifications serve as electrochemical labels that are detectable by either oxidation or reduction, respectively (see representation of the ON double strand and redox curves). The redox potentials of the labels differ depending on the nucleobase and respond to incorporation into ONs. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2059 / 2062

页数：4

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