Overexpression of the rFCA RNA recognition motif affects morphologies modifications in rice (Oryza sativa L.)

被引：15

作者：

Hong, Fang ^{[1
]}

Attia, Kotb ^{[1
]}

Wei, Chun ^{[1
]}

Li, Kegui ^{[1
]}

He, Guangming ^{[1
]}

Su, Wei ^{[1
]}

Zhang, Qinghua ^{[1
]}

Qian, Xiaoyin ^{[1
]}

Yang, Jinshui ^{[1
]}

机构：

[1] Fudan Univ, Sch Life Sci, Inst Genet, State Key Lab Genet Engn, Shanghai 200433, Peoples R China

来源：

BIOSCIENCE REPORTS | 2007年 / 27卷 / 4-5期

关键词：

FCA; RRM; rice (Oryza sativa L.); cell size;

D O I：

10.1007/s10540-007-9047-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA recognition motifs as important regulators of gene expression are highly conserved in animals and plants. The FCA floral promotion gene in Arabidopsis encodes a protein, containing two RNA recognition motifs (RRM) and a WW protein interaction domain. Here we isolated FCA cDNA from rice. FCA in rice (rFCA) was homologous to FCA-gamma of Arabidopsis and contained conserved domains. To investigate the function of RRM domain, fragment RRM1 and RRM2 of rFCA were introduced into rice subspecies Oryza sativa L. subsp. Indica var. 9311 and another rice subspecies Oryza sativa L. subsp. Japonica var. zhonghua11 transformation. Two transgenic lines exhibited similar phenotypes, flowering time delay, seed size and cell volume of transgenic plants was increased. These results showed that constitutive expression of RRMs could regulate cellular size. The patterns of overexpression of two RRM domains and their similar morphologies indicate they may play a same role.

引用

页码：225 / 234

页数：10

共 26 条

[1] Transformation and functional expression of the rFCA-RRM2 gene in rice [J].

Attia, K ;

Li, KG ;

Wei, C ;

He, GM ;

Su, W ;

Yang, JS .

JOURNAL OF INTEGRATIVE PLANT BIOLOGY, 2005, 47 (07) :823-830

[2] CONSERVED STRUCTURES AND DIVERSITY OF FUNCTIONS OF RNA-BINDING PROTEINS [J].

BURD, CG ;

DREYFUSS, G .

SCIENCE, 1994, 265 (5172) :615-621

[3] Posttranscriptional control of plant development [J].

Cheng, YL ;

Chen, XM .

CURRENT OPINION IN PLANT BIOLOGY, 2004, 7 (01) :20-25

[4] Alternative splicing and expression analysis of OsFCA (FCA in Oryza sativa L.), a gene homologous to FCA in Arabidopsis [J].

Du, Xiling ;

Qian, Xiaoyin ;

Wang, Dong ;

Yang, Jinshui .

DNA SEQUENCE, 2006, 17 (01) :31-40

[5] RNA-binding proteins in plants: the tip of an iceberg? [J].

Fedoroff, NV .

CURRENT OPINION IN PLANT BIOLOGY, 2002, 5 (05) :452-459

[6] Control of Arabidopsis flowering:: the chill before the bloom [J].

Henderson, IR ;

Dean, C .

DEVELOPMENT, 2004, 131 (16) :3829-3838

[7] Multiple domains control the subcellular localization and activity of ETR-3, a regulator of nuclear and cytoplasmic RNA processing events [J].

Ladd, AN ;

Cooper, TA .

JOURNAL OF CELL SCIENCE, 2004, 117 (16) :3519-3529

[8] atSRp30, one of two SF2/ASF-like proteins from Arabidopsis thaliana, regulates splicing of specific plant genes [J].

Lopato, S ;

Kalyna, M ;

Dorner, S ;

Kobayashi, R ;

Krainer, AR ;

Barta, A .

GENES & DEVELOPMENT, 1999, 13 (08) :987-1001

[9] Genome analysis:: RNA recognition motif (RRM) and K homology (KH) domain RNA-binding proteins from the flowering plant Arabidopsis thaliana [J].

Lorkovic, ZJ ;

Barta, A .

NUCLEIC ACIDS RESEARCH, 2002, 30 (03) :623-635

[10]

LOU M, 2005, P NATL ACAD SCI USA, V102, P17531

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