Development of a multiplex PCR detection of Salmonella spp. and Shigella spp. in mussels

被引：45

作者：

Vantarakis, A ^{[1
]}

Komninou, G ^{[1
]}

Venieri, D ^{[1
]}

Papapetropoulou, M ^{[1
]}

机构：

[1] Univ Patras, Sch Med, Publ Hlth Lab, GR-26110 Patras, Greece

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2000年 / 31卷 / 02期

关键词：

D O I：

10.1046/j.1365-2672.2000.00797.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Multiplex PCR amplification of invA and virA genes was developed enabling simultaneous detection in mussels of Salmonella spp, and Shigella spp., respectively. Simultaneous amplification of products of 215 and 275 bp was obtained either by using mixtures of individual strains of Sh. dysenteriae and Salm. typhimurium or spiked cont aminated mussels with both bacteria. In the case of the mussels, 10-100 cells of Salmonella spp. and Shigella per millilitre of homogenate were detected by the multiples PCR following a pre-enrichment step to increase sensitivity and to ensure that detection was based on the presence of cultivable bacteria. Also, the sensitivity and specificity of this method was evaluated. Multiplex PCR amplification was shown to be an effective, sensitive and rapid method for the simultaneous detection of pathogens in mussels.

引用

页码：105 / 109

页数：5

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