Fabrication and characterization of a sponge-like asymmetric chitosan membrane as a wound dressing

被引:579
作者
Mi, FL
Shyu, SS [1 ]
Wu, YB
Lee, ST
Shyong, JY
Huang, RN
机构
[1] Natl Cent Univ, Dept Chem Engn, Lab Polymer Mat Res, Chungli 32054, Taiwan
[2] Chinese Naval Acad, Div Chem, Dept Math Phys & Chem, Kaohsiung 813, Taiwan
[3] Naval 806 Hosp, Dept Surg & Plast Surg, Kaohsiung 813, Taiwan
[4] Natl Cent Univ, Inst Life Sci, Chungli 320, Taiwan
关键词
chitosan; asymmetric membrane; wound dressing; immersion-precipitation phase inversion;
D O I
10.1016/S0142-9612(00)00167-8
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
A novel asymmetric chitosan membrane has been prepared by immersion-precipitation phase-inversion method and evaluated as wound covering. This new type of chitosan wound dressing which consists of skin surface on top-layer supported by a macroporous sponge-like sublayer was designed. The thickness of the dense skin surface and porosity of sponge-like sublayer could be controlled by the modification of phase-separation process using per-evaporation method. The asymmetric chitosan membrane showed controlled evaporative water loss, excellent oxygen permeability and promoted fluid drainage ability but could inhibit exogenous microorganisms invasion due to the dense skin layer and inherent antimicrobial property of chitosan. Wound covered with the asymmetric chitosan membrane was hemostatic and healed quickly. Histological examination confirmed that epithelialization rate was increased and the deposition of collagen in the dermis was well organized by covering the wound with this asymmetric chitosan membrane. The results in this study indicate that the asymmetric chitosan membrane thus prepared could be adequately employed in the future as a wound dressing. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:165 / 173
页数:9
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