Colloidal gold and colloidal gold labelled wheat germ agglutinin as molecular probes for identification in mucin/chitosan complexes

被引:18
作者
Fiebrig, I
Varum, KM
Harding, SE
Davis, SS
Stokke, BT [1 ]
机构
[1] Norwegian Univ Sci & Technol, Dept Phys, NOBIPOL, NTNU, N-7034 Trondheim, Norway
[2] Univ Nottingham, Dept Pharmaceut Sci, Nottingham NG7 2RD, England
[3] Norwegian Univ Sci & Technol, NTNU, Dept Biotechnol, NOBIPOL, N-7034 Trondheim, Norway
[4] Univ Nottingham, Natl Ctr Macromol Hydrodynam, Sutton LE12 5RD, Surrey, England
关键词
D O I
10.1016/S0144-8617(97)00028-3
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Polyelectrolyte complex formation between pig gastric mucin and chitosan and its implications to mucoadhesion have recently been studied using techniques such as analytical ultracentrifugation, static light scattering and turbidimetry (Fiebrig, I., Harding, S.E. and Davis, S.S. (1994) Frog. Cell. Polym. Sci. 93, 66-73; Fiebrig, I., Harding, S.E., Rowe, A.J., Hyman, S.C. and Davies, S.S. (1995) Carbohyd. Polym. 28, 239-44). The findings suggested the formation of large complexes, which were then visualised using electron microscopy. The present investigation employed electron microscopy combined with colloidal gold conjugates in order to identify and localize chitosan within the mucin/chitosan complex. Images of apparently dispersed chitosan molecules could be obtained using an anionic dye molecule, Orange IJ, which is known to interact stoichiometrically with the cationic sites on chitosan. Once chitosan was complexed with mucin, the colloidal gold labelled techniques revealed that chitosan is concentrated in the centre of the complex, surrounded by a possibly more hydrophilic layer of mucin. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码:91 / 99
页数:9
相关论文
共 35 条
[1]  
ALLEN A, 1989, HDB PHYSL GASTROINTE, V3, P359
[2]   PURIFICATION, COMPOSITION AND SPECIFICITY OF WHEAT-GERM AGGLUTININ [J].
ALLEN, AK ;
NEUBERGER, A ;
SHARON, N .
BIOCHEMICAL JOURNAL, 1973, 131 (01) :155-162
[3]  
Anderson M. T., 1991, THESIS U NOTTINGHAM
[4]   SOLUTION PROPERTIES OF CHITOSANS - CONFORMATION AND CHAIN STIFFNESS OF CHITOSANS WITH DIFFERENT DEGREES OF N-ACETYLATION [J].
ANTHONSEN, MW ;
VARUM, KM ;
SMIDSROD, O .
CARBOHYDRATE POLYMERS, 1993, 22 (03) :193-201
[5]   AGGREGATES IN ACIDIC SOLUTIONS OF CHITOSANS DETECTED BY STATIC LASER-LIGHT SCATTERING [J].
ANTHONSEN, MW ;
VARUM, KM ;
HERMANSSON, AM ;
SMIDSROD, O ;
BRANT, DA .
CARBOHYDRATE POLYMERS, 1994, 25 (01) :13-23
[6]  
DEMEY J, 1984, EMSA B, V14, P54
[8]   HYDRODYNAMIC CHARACTERIZATION OF CHITOSANS VARYING IN DEGREE OF ACETYLATION [J].
ERRINGTON, N ;
HARDING, SE ;
VARUM, KM ;
ILLUM, L .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1993, 15 (02) :113-117
[9]  
Fiebrig I., 1994, European Journal of Pharmaceutical Sciences, V2, P185, DOI 10.1016/0928-0987(94)90438-3
[10]  
FIEBRIG I, 1994, PROG COLL POL SCI S, V94, P66