The Echinococcus granulosus antigen B shows a high degree of genetic variability

被引：55

作者：

Arend, AC

Zaha, A

Ayala, FJ

Haag, KL ^{[1
]}

机构：

[1] Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92697 USA

[2] Univ Fed Rio Grande do Sul, Programa Pos Grad Genet & Biol Mol, BR-91501970 Porto Alegre, RS, Brazil

[3] Univ Fed Rio Grande do Sul, Ctr Biotecnol, BR-91501970 Porto Alegre, RS, Brazil

[4] Univ Fed Rio Grande do Sul, Dept Genet, BR-91501970 Porto Alegre, RS, Brazil

来源：

EXPERIMENTAL PARASITOLOGY | 2004年 / 108卷 / 1-2期

关键词：

Echinococcus granulosus; antigen B; genetic variability; cloning; sequencing;

D O I：

10.1016/j.exppara.2004.07.009

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Echinococcus granulosus larvae secret a polymeric lipoprotein known as antigen B (AgB) into the metacestode hydatid fluid. Three similar AgB subunits have been previously identified (AgB1, AgB32, and AgB3), and their respective genes isolated, but the actual number of genes encoding AgB subunits remains uncertain. In this study, we characterize the variability of genes encoding the AgB2 subunit, using PCR and RT-PCR followed by cloning and sequencing. We have analyzed 32 cDNA and 34 genomic sequences from a single metacestode, showing a high degree of sequence polymorphism. In addition, we have identified a possibly new AgB subunit, which we call AgB4. Additionally, we describe an AgB2 genomic clone lacking (i) a segment corresponding to the intron and (ii) a short, 45 bp sequence within exon II. The 45 bp segment encompasses the conserved splicing signals and corresponds to a highly conserved insect promoter motif. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：76 / 80

页数：5

共 20 条

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