Optical sensor for interstitial pH measurements

被引：20

作者：

Baldini, Francesco ^{[1
]}

Giannetti, Ambra ^{[1
]}

Mencaglia, Andrea A. ^{[1
]}

机构：

[1] CNR, Inst Appl Phys Nello Corrara, I-50019 Florence, Italy

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2007年 / 12卷 / 02期

关键词：

pH; optical sensor; interstitial fluid; microdialysis;

D O I：

10.1117/1.2714807

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An optical fiber sensor for measuring the pH in interstitial fluid is described. Microdialysis is the approach followed for extracting the sample from the subcutaneous adipose tissue. The interstitial fluid drawn flows through a microfluidic circuit formed by a microdialysis catheter in series with a pH glass capillary. The pH indicator (phenol red) is covalently immobilized on the internal wall of the glass capillary. An optoelectronic unit that makes use of LEDs and photodetectors is connected to the sensing capillary by means of optical fibers. Optical fibers are used to connect the interrogating unit to the sensing capillary. A resolution of 0.03 pH units and an accuracy of 0.07 pH units are obtained. Preliminary in vivo tests are carried out in pigs with altered respiratory function. (C) 2007 Society of Photo-Optical Instrumentation Engineers.

引用

页数：7

共 29 条

[1]

Arend M, 1998, ANGEW CHEM INT EDIT, V37, P1044, DOI 10.1002/(SICI)1521-3773(19980504)37:8<1044::AID-ANIE1044>3.0.CO

[2]

2-E

[3] Critical review of pH sensing with optical fibres [J].

Baldini, F .

CHEMICAL, BIOCHEMICAL, AND ENVIRONMENTAL FIBER SENSORS X, 1999, 3540 :2-9

[4]

BALDINI F, 2005, ADV ENV CHEM BIOL SE, V5993, P99309

[5] Glucose measurement in patients with diabetes mellitus with dermal interstitial fluid [J].

Bantle, JP ;

Thomas, W .

JOURNAL OF LABORATORY AND CLINICAL MEDICINE, 1997, 130 (04) :436-441

[6] Glucose monitoring with long-term subcutaneous microdialysis in neonates [J].

Baumeister, FAM ;

Rolinski, B ;

Busch, R ;

Emmrich, P .

PEDIATRICS, 2001, 108 (05) :1187-1192

[7] Universal buffer soluttons and the association constant of veronal [J].

Britton, HTS ;

Robinson, RA .

JOURNAL OF THE CHEMICAL SOCIETY, 1931, :1456-1462

[8] Optical pH, oxygen and carbon dioxide monitoring using a microdialysis approach [J].

Cooney, CG ;

Towe, BC ;

Eyster, CR .

SENSORS AND ACTUATORS B-CHEMICAL, 2000, 69 (1-2) :183-188

[9] Measurement of glucose and metabolites in subcutaneous adipose tissue during hyperglycemia with microdialysis at various perfusion flow rates [J].

Ekberg, NR ;

Wisniewski, N ;

Brismar, K ;

Ungerstedt, U .

CLINICA CHIMICA ACTA, 2005, 359 (1-2) :53-64

[10] Clinical evaluation of alternative-site glucose measurements in patients after major cardiac surgery [J].

Ellmerer, M ;

Haluzik, M ;

Blaha, J ;

Kremen, J ;

Svacina, S ;

Toller, W ;

Mader, J ;

Schaupp, L ;

Plank, J ;

Pieber, T .

DIABETES CARE, 2006, 29 (06) :1275-1281

← 1 2 3 →