CUG repeats present in myotonin kinase RNA form metastable "slippery" hairpins

被引：134

作者：

Napierala, M ^{[1
]}

Krzyzosiak, WJ ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Bioorgan Chem, Canc Genet Lab, PL-61704 Poznan, Poland

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1997年 / 272卷 / 49期

关键词：

D O I：

10.1074/jbc.272.49.31079

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show that CUG repeats form "slippery" hairpins in their natural sequence context of the myotonin kinase gene transcript. This novel type of RNA structure is characterized by strong S-1 and T-1 nuclease and lead cleavages in the terminal loop and by mild lead cleavages in the hairpin stem. The latter effect indicates a relaxed metastable structure of the stem. (CUG)(5) repeats do not form any detectable secondary structure, whereas hairpins of increasing stability are formed by (CUG)(11), (CUG)(21), and (CUG)(49). The potential role of the RNA hairpin structure in the pathogenesis of myotonic dystrophy is discussed.

引用

页码：31079 / 31085

页数：7

共 38 条

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