Vaccinia virus infection disrupts microtubule organization and centrosome function

被引：137

作者：

Ploubidou, A ^{[1
]}

Moreau, V ^{[1
]}

Ashman, K ^{[1
]}

Reckmann, I ^{[1
]}

González, C ^{[1
]}

Way, M ^{[1
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

来源：

EMBO JOURNAL | 2000年 / 19卷 / 15期

关键词：

centrosome; MAPs; microtubules; vaccinia virus;

D O I：

10.1093/emboj/19.15.3932

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the role of the microtubule cytoskeleton during vaccinia virus infection. We found that newly assembled virus particles accumulate in the vicinity of the microtubule-organizing centre in a microtubule- and dynein-dynactin complex-dependent fashion. Microtubules are required for efficient intracellular mature virus (IMV) formation and are essential for intracellular enveloped virus (IEV) assembly. As infection proceeds, the microtubule cytoskeleton becomes dramatically reorganized in a fashion reminiscent of overexpression of microtubule-associated proteins (MAPs). Consistent with this, we report that the vaccinia proteins A10L and L4R have MAP-like properties and mediate direct binding of viral cores to microtubules in vitro. In addition, vaccinia infection also results in severe reduction of proteins at the centrosome and loss of centrosomal microtubule nucleation efficiency. This represents the first example of viral-induced disruption of centrosome function. Further studies with vaccinia will provide insights into the role of microtubules during viral pathogenesis and regulation of centrosome function.

引用

页码：3932 / 3944

页数：13

共 79 条

[51] Characterization of vaccinia virus intracellular cores: Implications for viral uncoating and core structure [J].