Induction of innate immunity by IL-2-expressing Salmonella confers protection against lethal infection

被引:18
作者
al-Ramadi, BK
Mustafa, N
AbouHaidar, M
Fernandez-Cabezudo, MJ
机构
[1] UAE Univ, Fac Med & Hlth Sci, Dept Med Microbiol, Al Ain, U Arab Emirates
[2] UAE Univ, Fac Med & Hlth Sci, Dept Biochem, Al Ain, U Arab Emirates
关键词
innate immunity; Salmonella; interleukin-2; NK cells; NO production;
D O I
10.1016/S0161-5890(03)00005-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Previously, we demonstrated that an attenuated Salmonella strain expressing IL-2 (designated GIDIL2) is cleared from the circulation at a much faster rate than the non-cytokine-expressing parental strain (designated BRD509). These findings suggested that IL-2 expression led to the rapid induction of innate immune responses that, in turn, accounted for the accelerated rate of bacterial clearance. In the present study, the mechanism by which this early antibacterial response is mediated was investigated. We demonstrate that as early as 2 h after infection with GIDIL2, but not BRD509, peritoneal excudate cells exhibited enhanced NK cytotoxic activity and upregulated NOS2 mRNA and NO production. This early response coincided with an enhancement of GIDIL2 clearance from the peritoneal cavity, first evidenced 22 It post-infection. Moreover, it conferred a high level of protection against virulent Salmonella challenge given within 16-20 h of GIDIL2 administration. These findings highlight the importance of innate immunity in the control of early bacterial proliferation and demonstrate the rapidity by which these responses are induced following bacterial entry. (C) 2003 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:763 / 770
页数:8
相关论文
共 36 条
[1]   Cytokine expression by attenuated intracellular bacteria regulates the immune response to infection:: the Salmonella model [J].
al-Ramadi, BK ;
Adeghate, E ;
Mustafa, N ;
Ponery, AS ;
Fernandez-Cabezudo, MJ .
MOLECULAR IMMUNOLOGY, 2002, 38 (12-13) :931-940
[2]   Influence of vector-encoded cytokines on anti-Salmonella immunity:: Divergent effects of interleukin-2 and tumor necrosis factor alpha [J].
al-Ramadi, BK ;
Al-Dhaheri, MH ;
Mustafa, N ;
Abouhaidar, M ;
Xu, DM ;
Liew, FY ;
Lukic, ML ;
Fernandez-Cabezudo, MJ .
INFECTION AND IMMUNITY, 2001, 69 (06) :3980-3988
[3]   Role of nitric oxide in host defense in murine salmonellosis as a function of its antibacterial and antiapoptotic activities [J].
Alam, MS ;
Akaike, T ;
Okamoto, S ;
Kubota, T ;
Yoshitake, J ;
Sawa, T ;
Miyamoto, Y ;
Tamura, F ;
Maeda, H .
INFECTION AND IMMUNITY, 2002, 70 (06) :3130-3142
[4]  
ALRAMADI BK, 1991, J IMMUNOL, V146, P2737
[5]   IMMUNOSUPPRESSION INDUCED BY NITRIC-OXIDE AND ITS INHIBITION BY INTERLEUKIN-4 [J].
ALRAMADI, BK ;
MEISSLER, JJ ;
HUANG, D ;
EISENSTEIN, TK .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1992, 22 (09) :2249-2254
[6]  
ALRAMADI BK, 1995, J IMMUNOL, V155, P662
[7]   Immunobiology of dendritic cells [J].
Banchereau, J ;
Briere, F ;
Caux, C ;
Davoust, J ;
Lebecque, S ;
Liu, YT ;
Pulendran, B ;
Palucka, K .
ANNUAL REVIEW OF IMMUNOLOGY, 2000, 18 :767-+
[8]  
Basse P H, 2001, Int Rev Immunol, V20, P439, DOI 10.3109/08830180109054416
[9]   INTERLEUKIN-2-INDUCED PROLIFERATION OF MURINE NATURAL-KILLER CELLS INVIVO [J].
BIRON, CA ;
YOUNG, HA ;
KASAIAN, MT .
JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 171 (01) :173-188
[10]   Salmonella-induced cell death:: apoptosis, necrosis or programmed cell death? [J].
Boise, LH ;
Collins, CM .
TRENDS IN MICROBIOLOGY, 2001, 9 (02) :64-67