Bifunctional combined aptamer for simultaneous separation and detection of thrombin

被引：40

作者：

Bing, Tao ^{[1
,2
]}

Liu, Xiangjun ^{[1
]}

Cheng, Xiaohong ^{[1
,2
]}

Cao, Zehui ^{[1
]}

Shangguan, Dihua ^{[1
]}

机构：

[1] Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Beijing 100190, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2010年 / 25卷 / 06期

关键词：

Aptamer; Biosensor; Protein detection; Separation; Thrombin; Streptavidin; STRANDED-DNA MOLECULES; FLUORESCENCE ANISOTROPY; HIGH-AFFINITY; RNA APTAMERS; SELECTION; BIND; LIGANDS; SENSORS; D(GGTTGGTGTGGTTGG); OLIGONUCLEOTIDES;

D O I：

10.1016/j.bios.2009.11.003

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Here we report on the construction and evaluation of a bifunctional combined aptamer (BCA) that consists of a DNA streptavidin-binding aptamer (SBA), a DNA thrombin-binding aptamer (TBA) and a fluorophore. The BCA adopts a new conformation that is very different from simply linking the conformations of the two individual aptamers together, so that it does not bind to streptavidin in the absence of thrombin. Binding of this novel DNA aptamer to streptavidin is triggered by the thrombin binding and depends on the concentration of thrombin. Meanwhile, fluorescence from the streptavidin captured BCA reflects the quantity of the target molecule in the sample. This aptamer combination strategy based on the SBA holds good potential for applications in simultaneous detection and separation of targets of aptamers or certain DNA and RNA targets. (c) 2009 Elsevier B.V. All rights reserved.

引用

页码：1487 / 1492

页数：6

共 36 条

[1] Design Variations for an Aptamer-Based DNA Nanodevice [J].

Beyer, Stefan ;

Dittmer, Wendy U. ;

Simmel, Friedrich C. .

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2005, 1 (01) :96-101

[2] SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN [J].

BOCK, LC ;

GRIFFIN, LC ;

LATHAM, JA ;

VERMAAS, EH ;

TOOLE, JJ .

NATURE, 1992, 355 (6360) :564-566

[3] Bimodal loop-loop interactions increase the affinity of RNA aptamers for HIV-1 RNA structures [J].

Boucard, D ;

Toulmé, JJ ;

Di Primo, C .

BIOCHEMISTRY, 2006, 45 (05) :1518-1524

[4] Aptamers come of age - at last [J].

Bunka, David H. J. ;

Stockley, Peter G. .

NATURE REVIEWS MICROBIOLOGY, 2006, 4 (08) :588-596

[5] Nuclease resistance of telomere-like oligonucleotides monitored in live cells by fluorescence anisotropy imaging [J].

Cao, ZH ;

Huang, CC ;

Tan, WH .

ANALYTICAL CHEMISTRY, 2006, 78 (05) :1478-1484

[6] A DNA-based machine that can cyclically bind and release thrombin [J].

Dittmer, WU ;

Reuter, A ;

Simmel, FC .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (27) :3550-3553

[7] Parallel analysis of two analytes in solutions or on surfaces by using a bifunctional aptamer: Applications for biosensing and logic gate operations [J].

Elbaz, Johann ;

Shlyahovsky, Bella ;

Li, Di ;

Willner, Itamar .

CHEMBIOCHEM, 2008, 9 (02) :232-239

[8] SELECTION INVITRO OF SINGLE-STRANDED-DNA MOLECULES THAT FOLD INTO SPECIFIC LIGAND-BINDING STRUCTURES [J].

ELLINGTON, AD ;

SZOSTAK, JW .

NATURE, 1992, 355 (6363) :850-852

[9] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].

ELLINGTON, AD ;

SZOSTAK, JW .

NATURE, 1990, 346 (6287) :818-822

[10] Detection of small organic analytes by fluorescing molecular switches [J].

Frauendorf, C ;

Jäschke, A .

BIOORGANIC & MEDICINAL CHEMISTRY, 2001, 9 (10) :2521-2524

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