Larger intercellular variation in (Q/R) editing of GluR6 than GluR5 revealed by single cell RT-PCR

被引：6

作者：

Christensen, KV

Dai, WM

Lambert, JDC

Egebjerg, J

机构：

[1] Aarhus Univ, Dept Biol Mol & Struct, DK-8000 Aarhus C, Denmark

[2] Aarhus Univ, Dept Physiol, DK-8000 Aarhus, Denmark

来源：

NEUROREPORT | 2000年 / 11卷 / 16期

关键词：

kainate receptors; RNA editing; single-cell RT-PCR;

D O I：

10.1097/00001756-200011090-00035

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

RNA editing of the pre-mRNA encoding the kainate receptor subtypes determines the Ca2+ permeability and the rectifying properties of the receptors in which these are assembled. GluR6 pre-mRNA contains three characterized editing sites: Q/R, I/V and the Y/C, whereas GluR5 pre-mRNA contains only the (Q/R) site. Single cell RT PCR was used on cultured cortical neurons to determine the relative expression and editing levels of the kainate receptor subunits encoding mRNA. The analysis showed a large intercellular variation in editing efficiency. The overall lower level of GluR5 editing, in the culture, compared to GluR6 editing is a result of an similar to 60% lower editing efficiency of GluR5 pre-mRNA, within single cells, compared with GluR6. NeuroReport 11:3577-3582 (C) 2000 Lippincott Williams & Wilkins.

引用

收藏

页码：3577 / 3582

页数：6

相关论文

共 27 条

[1] Permeation and block of rat GluR6 glutamate receptor channels by internal and external polyamines [J].

Bahring, R ;

Bowie, D ;

Benveniste, M ;

Mayer, ML .

JOURNAL OF PHYSIOLOGY-LONDON, 1997, 502 (03) :575-589

[2] SYNTHETIC POLYNUCLEOTIDES AND AMINO ACID CODE .5. [J].

BASILIO, C ;

WAHBA, AJ ;

OCHOA, S ;

SPEYER, JF ;

LENGYEL, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1962, 48 (04) :613-&

[3] Characterization of RNA editing of the glutamate-receptor subunits GluR5 and GluR6 in granule cells during cerebellar development [J].

Belcher, SM ;

Howe, JR .

MOLECULAR BRAIN RESEARCH, 1997, 52 (01) :130-138

[4] Q/R editing of the rat GluR5 and GluR6 kainate receptors in vivo and in vitro:: evidence for independent developmental, pathological and cellular regulation [J].

Bernard, A ;

Ferhat, L ;

Dessi, F ;

Charton, G ;

Represa, A ;

Ben-Ari, Y ;

Khrestchatisky, M .

EUROPEAN JOURNAL OF NEUROSCIENCE, 1999, 11 (02) :604-616

[5] CLONING OF A NOVEL GLUTAMATE RECEPTOR SUBUNIT, GLUR5 - EXPRESSION IN THE NERVOUS-SYSTEM DURING DEVELOPMENT [J].

BETTLER, B ;

BOULTER, J ;

HERMANSBORGMEYER, I ;

OSHEAGREENFIELD, A ;

DENERIS, ES ;

MOLL, C ;

BORGMEYER, U ;

HOLLMANN, M ;

HEINEMANN, S .

NEURON, 1990, 5 (05) :583-595

[6] FRACTIONAL CALCIUM CURRENTS THROUGH RECOMBINANT GLUR CHANNELS OF THE NMDA, AMPA AND KAINATE RECEPTOR SUBTYPES [J].

BURNASHEV, N ;

ZHOU, Z ;

NEHER, E ;

SAKMANN, B .

JOURNAL OF PHYSIOLOGY-LONDON, 1995, 485 (02) :403-418

[7] Kainate receptors mediate a slow postsynaptic current in hippocampal CA3 neurons [J].

Castillo, PE ;

Malenka, RC ;

Nicoll, RA .

NATURE, 1997, 388 (6638) :182-186

[8] GluR5 kainate receptor activation in interneurons increases tonic inhibition of pyramidal cells [J].

Cossart, R ;

Esclapez, M ;

Hirsch, JC ;

Bernard, C ;

Ben-Ari, Y .

NATURE NEUROSCIENCE, 1998, 1 (06) :470-478

[9] CA2+ PERMEABILITY OF UNEDITED AND EDITED VERSIONS OF THE KAINATE SELECTIVE GLUTAMATE RECEPTOR GLUR6 [J].

EGEBJERG, J ;

HEINEMANN, SF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (02) :755-759

[10] CLONING OF A CDNA FOR A GLUTAMATE RECEPTOR SUBUNIT ACTIVATED BY KAINATE BUT NOT AMPA [J].

EGEBJERG, J ;

BETTLER, B ;

HERMANSBORGMEYER, I ;

HEINEMANN, S .

NATURE, 1991, 351 (6329) :745-748