Identification of impurities in erythromycin by liquid chromatography-mass spectrometric detection

被引：29

作者：

Chitneni, SK ^{[1
]}

Govaerts, C ^{[1
]}

Adams, E ^{[1
]}

Van Schepdael, A ^{[1
]}

Hoogmartens, J ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Fac Farmaceut Wetenschappen, Lab Farmaceut Chem Anal Geneesmiddelen, B-3000 Louvain, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1056卷 / 1-2期

关键词：

erythromycin; evaporative light scattering detection;

D O I：

10.1016/j.chroma.2004.07.091

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, isocratic liquid chromatographic (LC) method using volatile mobile phase constituents for the identification of related substances in erythromycin samples is described. For method development, evaporative light scattering detection (ELSD) was used. An XTerra(TM)RP18 column was used at 70 degreesC with a mobile phase consisting of acetonitrile-isopropanol-0.2 M ammonium acetate pH 7.0-water (165:105:50:680). Mass spectral data were acquired on an ion trap mass spectrometer equipped with an electrospray interface operated in the positive ion mode. First, a library was created using MS/MS and MSn spectra of reference substances available in the laboratory. Using these reference spectra as interpretative templates, eight novel related substances in erythromycin samples were identified: N-demethylerythromycin E, erythromycin E N-oxide, anhydroerythromycin C, N-demethylerythromycin B, anhydro-N-demethylerythromycin A, pseudoerythromycin E enol ether, EF lacking the neutral sugar and EA lacking the neutral sugar. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：111 / 120

页数：10

共 34 条

[1]

[Anonymous], 2003, US PHARM, P727

[2] KINETIC-STUDIES ON THE DECOMPOSITION OF ERYTHROMYCIN-A IN AQUEOUS ACIDIC AND NEUTRAL BUFFERS [J].