Characterization of coagulase-negative staphylococci isolated from patients with infected hip prostheses:: use of phenotypic and genotypic analyses, including tests for the presence of the ica operon

被引:22
作者
Nilsdotter-Augustinsson, A. [1 ]
Koskela, A.
Oehman, L.
Soederquist, B.
机构
[1] Linkoping Univ, Div Infect Dis, Dept Mol & Clin Med, S-58185 Linkoping, Sweden
[2] Linkoping Univ, Div Med Microbiol, Dept Mol & Clin Med, S-58185 Linkoping, Sweden
[3] Orebro Univ Hosp, Clin Res Ctr, S-70185 Orebro, Sweden
[4] Orebro Univ Hosp, Dept Clin Microbiol, S-70185 Orebro, Sweden
关键词
D O I
10.1007/s10096-007-0281-9
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The aim of this study was to investigate phenotypic and/or genotypic heterogeneity in coagulase-negative staphylococci (CoNS) obtained from multiple tissue samples taken perioperatively during exchange surgery from each of 19 patients with clinically and/or microbiologically proven hip prosthesis infections. CoNS are important pathogens in prosthetic hip joint infections. Several virulence factors have been suggested for CoNS, such as phenotypic variation, yet the pathogenic processes that are involved remain unclear. The PhenePlate system (PhPlate AB, Stockholm Sweden) was used for phenotyping and pulsed-field gel electrophoresis for genotyping of polymorphisms in isolates of CoNS. Furthermore, polymerase chain reaction was used to determine the presence of the icaADB gene complex in the isolates. Some patients were infected with CoNS and other species, some were infected with multiple CoNS species, although infections with Staphylococcus epidermidis alone were most common, and some were infected with different S. epidermidis clones. Phenotypic variation was found among isolates both from the same tissue sample and from different samples from the same patient, and in some cases such variation represented the presence of different clones. One-third of the patients infected with S. epidermidis carried the icaADB genes. CoNS isolates showing phenotypic and/or genotypic heterogeneity were identified in tissue samples from half of the patients. The presence of the intercellular adhesion (ica) operon does not seem to be a prerequisite for establishing infection with CoNS.
引用
收藏
页码:255 / 265
页数:11
相关论文
共 53 条
[51]   A novel mechanism of phase variation of virulence in Staphylococcus epidermidis:: evidence for control of the polysaccharide intercellular adhesin synthesis by alternating insertion and excision of the insertion sequence element IS256 [J].
Ziebuhr, W ;
Krimmer, V ;
Rachid, S ;
Lössner, I ;
Götz, F ;
Hacker, J .
MOLECULAR MICROBIOLOGY, 1999, 32 (02) :345-356
[52]   Detection of the intercellular adhesion gene cluster (ica) and phase variation in Staphylococcus epidermidis blood culture strains and mucosal isolates [J].
Ziebuhr, W ;
Heilmann, C ;
Gotz, F ;
Meyer, P ;
Wilms, K ;
Straube, B ;
Hacker, J .
INFECTION AND IMMUNITY, 1997, 65 (03) :890-896
[53]   Current concepts: Prosthetic-joint infections [J].
Zimmerli, W ;
Trampuz, A ;
Ochsner, PE .
NEW ENGLAND JOURNAL OF MEDICINE, 2004, 351 (16) :1645-1654