Genetic Determinants of Differential Oral Infection Phenotypes of West Nile and St. Louis Encephalitis Viruses in Culex spp. Mosquitoes

被引:9
作者
Maharaj, Payal D. [2 ,3 ]
Bolling, Bethany G. [1 ,4 ]
Anishchenko, Michael [1 ]
Reisen, William K. [2 ,3 ]
Brault, Aaron C. [1 ]
机构
[1] Ctr Dis Control & Prevent, Div Vector Borne Dis, Ft Collins, CO 80521 USA
[2] Univ Calif Davis, Ctr Vectorborne Dis, Davis, CA 95616 USA
[3] Univ Calif Davis, Sch Vet Med, Dept Pathol Microbiol & Immunol, Davis, CA 95616 USA
[4] Univ Texas Med Branch, Galveston, TX 77555 USA
基金
美国国家卫生研究院;
关键词
CULICIDAE VECTOR COMPETENCE; NEW-YORK; DISSEMINATION RATES; VIRAL DISSEMINATION; DISEASE VECTOR; DIPTERA; QUINQUEFASCIATUS; PROTEIN; REPLICATION; EXPRESSION;
D O I
10.4269/ajtmh.14-0289
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
St. Louis encephalitis virus (SLEV) has shown greater susceptibility to oral infectivity than West Nile virus (WNV) in Culex mosquitoes. To identify the viral genetic elements that modulate these disparate phenotypes, structural chimeras (WNV-pre-membrane [prM] and envelope [E] proteins [prME]/SLEV.IC (infectious clone) and SLEV-prME/WNV.IC) were constructed in which two of the structural proteins, the prM and E, were interchanged between viruses. Oral dose-response assessment with the chimeric/parental WNV and SLEV was performed to characterize the infection phenotypes in Culex mosquitoes by artificial blood meals. The median infectious dose required to infect 50% of Cx. quinquefasciatus with WNV was indistinguishable from that of the SLEV-prME/WNV.IC chimeric virus. Similarly, SLEV and WNV-prME/SLEV.IC virus exhibited an indistinguishable oral dose-response relationship in Cx. quinquefasciatus. Infection rates for WNV.IC and SLEV-prME/WNV.IC were significantly lower than SLEV.IC and WNV-prME/SLEV.IC infection rates. These results indicated that WNV and SLEV oral infectivities are not mediated by genetic differences within the prM and E proteins.
引用
收藏
页码:1066 / 1072
页数:7
相关论文
共 57 条
[1]  
[Anonymous], PLOS NEGL TROP DIS
[2]   Envelope protein glycosylation status influences mouse neuroinvasion phenotype of genetic lineage 1 West Nile Virus strains [J].
Beasley, DWC ;
Whiteman, MC ;
Zhang, SL ;
Huang, CYH ;
Schneider, BS ;
Smith, DR ;
Gromowski, GD ;
Higgs, S ;
Kinney, RM ;
Barrett, ADT .
JOURNAL OF VIROLOGY, 2005, 79 (13) :8339-8347
[3]  
Beaty BJ., 1995, DIAGNOSTIC PROCEDURE, V7th, P189
[4]  
BELLAMY R. E., 1958, MOSQUITO NEWS, V18, P132
[5]   Variation in vector competence for dengue 2 virus among 24 collections of Aedes aegypti from Mexico and the United States [J].
Bennett, KE ;
Olson, KE ;
Muñoz, MD ;
Fernandez-Salas, I ;
Farfan-Ale, JA ;
Higgs, S ;
Black, WC ;
Beaty, BJ .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2002, 67 (01) :85-92
[6]  
Bernard KA, 2001, EMERG INFECT DIS, V7, P679
[7]  
Blair CD, 2011, FUTURE MICROBIOL, V6, P265, DOI [10.2217/fmb.11.11, 10.2217/FMB.11.11]
[8]  
Blitvich Bradley J., 2008, Animal Health Research Reviews, V9, P71, DOI 10.1017/S1466252307001430
[9]   RNAi Targeting of West Nile Virus in Mosquito Midguts Promotes Virus Diversification [J].
Brackney, Doug E. ;
Beane, Jennifer E. ;
Ebel, Gregory D. .
PLOS PATHOGENS, 2009, 5 (07)
[10]   Adaptation of two flaviviruses results in differences in genetic heterogeneity and virus adaptability [J].
Ciota, Alexander T. ;
Lovelace, Amy O. ;
Jones, Susan A. ;
Payne, Anne ;
Kramer, Laura D. .
JOURNAL OF GENERAL VIROLOGY, 2007, 88 :2398-2406