Bre1 is required for notch signaling and histone modification

被引:101
作者
Bray, S [1 ]
Musisi, H
Bienz, M
机构
[1] Univ Cambridge, Dept Anat, Cambridge, England
[2] MRC, Mol Biol Lab, Cambridge CB2 2QH, England
基金
英国医学研究理事会;
关键词
D O I
10.1016/j.devcel.2004.11.020
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Notch signaling controls numerous cell fate decisions during animal development. These typically involve a Notch-mediated switch in transcription of target genes, although the details of this molecular mechanism are poorly understood. Here, we identify dBre1 as a nuclear component required cell autonomously for the expression of Notch target genes in Drosophila development. dBre1 affects the levels of Su(H) in imaginal disc cells, and it stimulates the Su(H)-mediated transcription of a Notch-specific reporter in transfected Drosophila cells. Strikingly, dBre1 mutant clones show much reduced levels of methylated lysine 4 on histone 3 (H3K4m), a chromatin mark that has been implicated in transcriptional activation. Thus, dBre1 is the functional homolog of yeast Bre1p, an E3 ubiquitin ligase required for the monoubiquitination of histone H2B and, indirectly, for H3K4 methylation. Our results indicate that histone modification is critical for the transcription of Notch target genes.
引用
收藏
页码:279 / 286
页数:8
相关论文
共 52 条
[1]   Notch signaling: Cell fate control and signal integration in development [J].
Artavanis-Tsakonas, S ;
Rand, MD ;
Lake, RJ .
SCIENCE, 1999, 284 (5415) :770-776
[2]   SUPPRESSOR OF HAIRLESS DIRECTLY ACTIVATES TRANSCRIPTION OF ENHANCER OF SPLIT COMPLEX GENES IN RESPONSE TO NOTCH RECEPTOR ACTIVITY [J].
BAILEY, AM ;
POSAKONY, JW .
GENES & DEVELOPMENT, 1995, 9 (21) :2609-2622
[3]   Evolution of proneural atonal expression during distinct regulatory phases in the developing Drosophila eye [J].
Baker, NE ;
Yu, S ;
Han, D .
CURRENT BIOLOGY, 1996, 6 (10) :1290-1301
[4]   PATTERNS OF EXPRESSION OF CUT, A PROTEIN REQUIRED FOR EXTERNAL SENSORY ORGAN DEVELOPMENT IN WILD-TYPE AND CUT MUTANT DROSOPHILA EMBRYOS [J].
BLOCHLINGER, K ;
BODMER, R ;
JAN, LY ;
JAN, YN .
GENES & DEVELOPMENT, 1990, 4 (08) :1322-1331
[5]  
BRAND AH, 1993, DEVELOPMENT, V118, P401
[6]   A non-proteolytic role for ubiquitin in Tat-mediated transactivation of the HIV-1 promoter [J].
Brès, V ;
Kiernan, RE ;
Linares, LK ;
Chable-Bessia, C ;
Plechakova, O ;
Tréand, C ;
Emiliani, S ;
Peloponese, JM ;
Jeang, KT ;
Coux, O ;
Scheffner, M ;
Benkirane, M .
NATURE CELL BIOLOGY, 2003, 5 (08) :754-761
[7]   Gastrointestinal stem cells [J].
Brittan, M ;
Wright, NA .
JOURNAL OF PATHOLOGY, 2002, 197 (04) :492-509
[8]   NOTCH IS REQUIRED FOR SUCCESSIVE CELL DECISIONS IN THE DEVELOPING DROSOPHILA RETINA [J].
CAGAN, RL ;
READY, DF .
GENES & DEVELOPMENT, 1989, 3 (08) :1099-1112
[9]  
Christensen S, 1996, DEVELOPMENT, V122, P1373
[10]  
deCelis JF, 1996, DEVELOPMENT, V122, P2719