Methicillin-Resistant Coagulase-Negative Staphylococci in the Community: High Homology of SCCmec IVa between Staphylococcus epidermidis and Major Clones of Methicillin-Resistant Staphylococcus aureus

被引:130
作者
Barbier, Francois [2 ,3 ,4 ]
Ruppe, Etienne [2 ,3 ]
Hernandez, David
Lebeaux, David [2 ,3 ]
Francois, Patrice [7 ]
Felix, Benjamin [6 ]
Desprez, Adeline [2 ,3 ]
Maiga, Aminata [3 ,8 ]
Woerther, Paul-Louis [2 ,3 ]
Gaillard, Kevin [2 ]
Jeanrot, Cecile [5 ]
Wolff, Michel [3 ,4 ]
Schrenzel, Jacques [7 ]
Andremont, Antoine [2 ,3 ]
Ruimy, Raymond [1 ,2 ,3 ]
机构
[1] Hop Bichat Claude Bernard, Bacteriol Lab, AP HP, Bacteriol Unit, F-75018 Paris, France
[2] Hop Bichat Claude Bernard, Natl Reference Ctr Emergence Resistance Commensal, AP HP, F-75018 Paris, France
[3] Hop Bichat Claude Bernard, EA 3964, AP HP, F-75018 Paris, France
[4] Hop Bichat Claude Bernard, Med Intens Care Unit, AP HP, F-75018 Paris, France
[5] Hop Bichat Claude Bernard, Orthoped & Trauma Surg Unit, AP HP, F-75018 Paris, France
[6] Natl Inst Agron Res, Jouy En Josas, France
[7] Univ Hosp Geneva, Genom Res Lab, Geneva, Switzerland
[8] Point G Hosp, Bacteriol Unit, Bamako, Mali
基金
瑞士国家科学基金会;
关键词
CASSETTE CHROMOSOME MEC; PANTON-VALENTINE LEUKOCIDIN; CATABOLIC MOBILE ELEMENT; GENOME SEQUENCE; USA300; CLONE; STRAINS; EMERGENCE; EVOLUTION; HAEMOLYTICUS; PREVALENCE;
D O I
10.1086/653483
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background. Data on community spread of methicillin-resistant coagulase-negative staphylococci (MR-CoNS) are scarce. We assessed their potential role as a reservoir of staphylococcal cassette chromosome mec (SCCmec) IVa, the leading SCCmec subtype in community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA). Methods. Nasal carriage of MR-CoNS was prospectively investigated in 291 adults at hospital admission. MR-CoNS were characterized by SCCmec typing, long-range polymerase chain reaction (PCR) for SCCmec IV, and multiple-locus variable-number tandem repeat analysis (MLVA) for Staphylococcus epidermidis (MRSE) strains. Three SCCmec IVa elements were fully sequenced. Results. The carriage rate of MR-CoNS was 19.2% (25.9% and 16.5% in patients with and patients without previous exposure to the health care system, respectively; P = .09). MR-CoNS strains (n = 83, including 58 MRSE strains with highly heterogeneous MLVA patterns) carried SCCmec type IVa (n = 9, all MRSE), other SCCmec IV subtypes (n = 9, including 7 MRSE), other SCCmec types (n = 15), and nontypeable SCCmec (n = 50). Long-range PCR indicated structural homology between SCCmec IV in MRSE and that in MRSA. Complete sequences of SCCmec IVa from 3 MRSE strains were highly homologous to those available for CA-MRSA, including major clones USA300 and USA400. Conclusions. MR-CoNS are probably disseminated in the community, notably in subjects without previous exposure to the health care system. MRSE, the most prevalent species, may act as a reservoir of SCCmec IVa for CA-MRSA.
引用
收藏
页码:270 / 281
页数:12
相关论文
共 51 条
[31]   Guiding empirical antibiotic therapy in orthopaedics: The microbiology of prosthetic joint infection managed by debridement, irrigation and prosthesis retention [J].
Moran, E. ;
Masters, S. ;
Berendt, A. R. ;
McLardy-Smith, P. ;
Byren, I. ;
Atkins, B. L. .
JOURNAL OF INFECTION, 2007, 55 (01) :1-7
[32]   Methicillin-resistant S-aureus infections among patients in the emergency department [J].
Moran, Gregory J. ;
Krishnadasan, Anusha ;
Gorwitz, Rachel J. ;
Fosheim, Gregory E. ;
McDougal, Linda K. ;
Carey, Roberta B. ;
Talan, David A. .
NEW ENGLAND JOURNAL OF MEDICINE, 2006, 355 (07) :666-674
[33]   Predominance of community-associated methicillin-resistant Staphylococcus aureus strains carrying staphylococcal chromosome Cassette mec type IVA in South Korea [J].
Park, Chulmin ;
Lee, Dong-Gun ;
Kim, Sun Woo ;
Choi, Su-Mi ;
Park, Sun Hee ;
Chun, Hye-Sun ;
Choi, Jung-Hyun ;
Yoo, Jin-Hong ;
Shin, Wan Shik ;
Kang, Jin Han ;
Kim, Jong Hyun ;
Lee, Soo Young ;
Kim, Sun Mi ;
Pyun, Bok Yang .
JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (12) :4021-4026
[34]   PHYLOGENETIC ANALYSIS AND ASSESSMENT OF THE GENERA VIBRIO, PHOTOBACTERIUM, AEROMONAS, AND PLESIOMONAS DEDUCED FROM SMALL-SUBUNIT RIBOSOMAL-RNA SEQUENCES [J].
RUIMY, R ;
BREITTMAYER, V ;
ELBAZE, P ;
LAFAY, B ;
BOUSSEMART, O ;
GAUTHIER, M ;
CHRISTEN, R .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1994, 44 (03) :416-426
[35]   Accuracy and potential usefulness of triplex real-time PCR for improving antibiotic treatment of patients with blood cultures showing clustered gram-positive cocci on direct smears [J].
Ruimy, Raymond ;
Dos-Santos, Marie ;
Raskine, Laurent ;
Bert, Frederic ;
Masson, Rene ;
Elbaz, Sandrine ;
Bonnal, Christine ;
Lucet, Jean-Christophe ;
Lefort, Agnes ;
Fantin, Bruno ;
Wolff, Michel ;
Hornstein, Michele ;
Andremont, Antoine .
JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (06) :2045-2051
[36]   Diversity of Staphylococcal Cassette Chromosome mec Structures in Methicillin-Resistant Staphylococcus epidermidis and Staphylococcus haemolyticus Strains among Outpatients from Four Countries [J].
Ruppe, Etienne ;
Barbier, Francois ;
Mesli, Yasmine ;
Maiga, Aminata ;
Cojocaru, Radu ;
Benkhalfat, Mokhtar ;
Benchouk, Samia ;
Hassaine, Hafida ;
Maiga, Ibrahim ;
Diallo, Amadou ;
Koumare, Abdel Karim ;
Ouattara, Kalilou ;
Soumare, Sambou ;
Dufourcq, Jean-Baptiste ;
Nareth, Chhor ;
Sarthou, Jean-Louis ;
Andremont, Antoine ;
Ruimy, Raymond .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (02) :442-449
[37]  
Silva FR, 2001, EPIDEMIOL INFECT, V127, P57, DOI 10.1017/S095026880100574X
[38]   Minimus: a fast, lightweight genome assembler [J].
Sommer, Daniel D. ;
Delcher, Arthur L. ;
Salzberg, Steven L. ;
Pop, Mihai .
BMC BIOINFORMATICS, 2007, 8
[39]   Emergence of SCCmec Type IV as the Most Common Type of Methicillin-Resistant Staphylococcus aureus in a University Hospital [J].
Stranden, A. M. ;
Frei, R. ;
Adler, H. ;
Flueckiger, U. ;
Widmer, A. F. .
INFECTION, 2009, 37 (01) :44-48
[40]   Novel characteristics of community-acquired methicillin-resistant Staphylococcus aureus strains belonging to multilocus sequence type 59 in Taiwan [J].
Takano, Tomomi ;
Higuchi, Wataru ;
Otsuka, Taketo ;
Baranovich, Tatiana ;
Enany, Shymaa ;
Saito, Kohei ;
Isobe, Hirokazu ;
Dohmae, Soshi ;
Ozaki, Kyoko ;
Takano, Nfisao ;
Iwao, Yasuhisa ;
Shibuya, Michiko ;
Okubo, Takeshi ;
Yabe, Shizuka ;
Shi, Da ;
Reva, Ivan ;
Teng, Lee-Jene ;
Yamamoto, Tatsuo .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2008, 52 (03) :837-845