The haemagglutinin of Clostridium botulinum type C progenitor toxin plays an essential role in binding of toxin to the epithelial cells of guinea pig small intestine, leading to the efficient absorption of the toxin

被引:111
作者
Fujinaga, Y
Inoue, K
Watanabe, S
Yokota, K
Hirai, Y
Nagamachi, E
Oguma, K
机构
[1] Okayama Univ, Sch Med, Dept Bacteriol, Okayama 700, Japan
[2] Kobe City Coll Nursing, Nishi Ku, Kobe, Hyogo 651, Japan
[3] Kibi Int Univ, Sch Hlth Sci, Dept Nursing, Okayama 716, Japan
来源
MICROBIOLOGY-UK | 1997年 / 143卷
关键词
haemagglutinin; Clostridium botulinum; toxin binding; absorption;
D O I
10.1099/00221287-143-12-3841
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Binding of the purified type C 7S (neurotoxin), 12S and 16S botulinum toxins to epithelial cells of ligated small intestine or colon of the guinea pig (in vivo test) and to pre-fixed gastrointestinal tissue sections (in vitro test) wasanalysed. The 165 toxin bound intensely to the microvilli of epithelial cells of the small intestine in both in vivo and in vitro tests, but did not bind to cells of the stomach or colon. The neurotoxin and 12S toxin did not bind to epithelial cells of the small intestine or to cells of the stomach or colon. Absorption of the toxins was assessed by determining the toxin titre in the sera of guinea pigs 6-8 h after the intra-intestinal administration of the toxins. When the 165 toxin [1 x 10(5) minimum lethal dose (MLD)] was injected, 200-660 MLD ml(-1) was detected in the sera, whereas when the 12S toxin (2 x 10(5) MLD) or 7S toxin (2 x 10(5) MLD) was injected, little toxin activity was detected in the sera. Therefore, the haemagglutinin of type C 16S toxin is apparently very important in the binding and absorption of botulinum toxin in the small intestine.
引用
收藏
页码:3841 / 3847
页数:7
相关论文
共 18 条
[1]   DISTRIBUTION AND CHANGES OF GLYCOCONJUGATES IN RAT COLONIC MUCOSA DURING DEVELOPMENT - A HISTOCHEMICAL-STUDY USING LECTINS [J].
CALDERO, J ;
CAMPO, E ;
CALOMARDE, X ;
TORRA, M .
HISTOCHEMISTRY, 1988, 90 (04) :261-270
[2]   MOLECULAR CONSTRUCTION OF CLOSTRIDIUM-BOTULINUM TYPE-C PROGENITOR TOXIN AND ITS GENE ORGANIZATION [J].
FUJINAGA, Y ;
INOUE, K ;
SHIMAZAKI, S ;
TOMOCHIKA, K ;
TSUZUKI, K ;
FUJII, N ;
WATANABE, T ;
OHYAMA, T ;
TAKESHI, K ;
INOUE, K ;
OGUMA, K .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 205 (02) :1291-1298
[3]   MOLECULAR CHARACTERIZATION OF 2 FORMS OF NONTOXIC-NONHEMAGGLUTININ COMPONENTS OF CLOSTRIDIUM-BOTULINUM TYPE-A PROGENITOR TOXINS [J].
FUJITA, R ;
FUJINAGA, Y ;
INOUE, K ;
NAKAJIMA, H ;
KUMON, H ;
OGUMA, K .
FEBS LETTERS, 1995, 376 (1-2) :41-44
[4]   ORGANIZATION OF THE BOTULINUM NEUROTOXIN C1 GENE AND ITS ASSOCIATED NONTOXIC PROTEIN GENES IN CLOSTRIDIUM-BOTULINUM-C-468 [J].
HAUSER, D ;
EKLUND, MW ;
BOQUET, P ;
POPOFF, MR .
MOLECULAR & GENERAL GENETICS, 1994, 243 (06) :631-640
[5]  
Henderson I, 1996, FEMS MICROBIOL LETT, V140, P151, DOI 10.1016/0378-1097(96)00172-3
[6]   Molecular composition of Clostridium botulinum type A progenitor toxins [J].
Inoue, K ;
Fujinaga, Y ;
Watanabe, T ;
Ohyama, T ;
Takeshi, K ;
Moriishi, K ;
Nakajima, H ;
Inoue, K ;
Oguma, K .
INFECTION AND IMMUNITY, 1996, 64 (05) :1589-1594
[7]   THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE GENE CODING FOR BOTULINUM TYPE-C1 TOXIN IN THE C-ST PHAGE GENOME [J].
KIMURA, K ;
FUJII, N ;
TSUZUKI, K ;
MURAKAMI, T ;
INDOH, T ;
YOKOSAWA, N ;
TAKESHI, K ;
SYUTO, B ;
OGUMA, K .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 171 (03) :1304-1311
[8]   STRUCTURE AND FUNCTION OF CLOSTRIDIUM-BOTULINUM TOXINS [J].
OGUMA, K ;
FUJINAGA, Y ;
INOUE, K .
MICROBIOLOGY AND IMMUNOLOGY, 1995, 39 (03) :161-168
[9]  
OGUMA K, 1980, INFECT IMMUN, V30, P656
[10]  
OHISHI I, 1980, INFECT IMMUN, V28, P303