Marker-assisted backcross introgression of the Yd2 gene conferring resistance to barley yellow dwarf virus in barley

被引：39

作者：

Jefferies, SP ^{[1
]}

King, BJ ^{[1
]}

Barr, AR ^{[1
]}

Warner, P ^{[1
]}

Logue, SJ ^{[1
]}

Langridge, P ^{[1
]}

机构：

[1] Univ Adelaide, Dept Plant Sci, Glen Osmond, SA 5064, Australia

来源：

PLANT BREEDING | 2003年 / 122卷 / 01期

关键词：

Hordeum vulgare; backcrossing; barley leaf scald; barley yellow dwarf virus; malting quality; marker assisted selection; Yd2;

D O I：

10.1046/j.1439-0523.2003.00752.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

YLM, a codominant polymerase chain reaction (PCR) marker linked to Yd2 , could substantially improve the precision and efficiency of barley yellow dwarf virus (BYDV) resistance breeding. The aim of this study was to assess the effectiveness of YLM in a marker-assisted introgression programme and to quantify associations between the presence of Yd2 and other agronomic and quality traits. The Yd2 gene was introgressed into a BYDV-susceptible background through two cycles of marker-assisted backcrossing. BC2 F-2 -derived lines, either carrying or not carrying the YLM allele associated with resistance, were compared in the presence and absence of BYDV. The YLM marker was shown to be effective in the introgression of Yd2 . Lines carrying the YLM allele associated with resistance produced significantly fewer leaf symptoms and showed a reduction in yield loss when infected with BYDV. There were no deleterious effects associated with the introgression of Yd2 on grain yield, grain size or malting quality. The implications of marker-assisted selection for Yd2 on barley improvement are discussed.

引用

页码：52 / 56

页数：5

共 31 条

[11]

Graner A, 1996, THEOR APPL GENET, V93, P421, DOI 10.1007/BF00223185

[12] The relationship between growth rate and the expression of tolerance to barley yellow dwarf virus in barley [J].

Jones, A. T. ;

Catherall, P. L. .

ANNALS OF APPLIED BIOLOGY, 1970, 65 (01) :137-145

[13]

KRIEG PA, 1987, METHOD ENZYMOL, V155, P397

[14]

Lister Richard M., 1995, P29

[15] DETECTION OF BARLEY YELLOW DWARF VIRUS BY ENZYME-LINKED IMMUNOSORBENT ASSAY [J].

LISTER, RM ;

ROCHOW, WF .

PHYTOPATHOLOGY, 1979, 69 (06) :649-654

[16]

MACLEOD LC, 1991, P 5 BARL TECHN S, P80

[17]

McClelland J.L., 1986, The appeal of parallel distributed processing, P3, DOI DOI 10.1016/B978-1-4832-1446-7.50010-8

[18]

PALIWAL YC, 1977, PHYTOPATHOL Z, V89, P25

[19] Development of YLM, a codominant PCR marker closely linked to the Yd2 gene for resistance to barley yellow dwarf disease [J].

Paltridge, NG ;

Collins, NC ;

Bendahmane, A ;

Symons, RH .

THEORETICAL AND APPLIED GENETICS, 1998, 96 (08) :1170-1177

[20]

Qualset C. O., 1984, Barley yellow dwarf, a proceedings of the workshop December 6-8, 1983 CIMMYT Mexico, sponsored by the United Nations Development Programme and CIMMYT, P72

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