Activation of inwardly rectifying K+ channels by GABA-B receptors expressed in Xenopus oocytes

被引:28
作者
Uezono, Y
Akihara, M
Kaibara, M
Kawano, C
Shibuya, I
Ueda, Y
Yanagihara, N
Toyohira, Y
Yamashita, H
Taniyama, K
Izumi, F
机构
[1] Univ Occupat & Environm Hlth, Dept Pharmacol, Sch Med, Kitakyushu, Fukuoka 807, Japan
[2] Univ Occupat & Environm Hlth, Dept Physiol 1, Sch Med, Kitakyushu, Fukuoka 807, Japan
[3] Nagasaki Univ, Sch Med, Dept Pharmacol 2, Nagasaki 852, Japan
关键词
baclofen; electrophysiology; GABA-B receptor; GIRK; heterologous expression; K+ channel; rat cerebellum; Xenopus oocyte;
D O I
10.1097/00001756-199803090-00004
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
IN Xenopus oocytes coinjected with poly(A)(+) RNA derived from the rat cerebellum and cRNAs for the cloned G protein-gated inwardly rectifying K+ channel (GIRK), GIRK1 and GIRK2, the GABA-B agonist baclofen elicited inwardly rectifying K+ currents. The inward KC currents elicited by baclofen were inhibited by the selective GABA-B antagonists 2-OH saclofen and CGP 35348, and by the GIRK inhibitor Ba2+. In contrast, baclofen caused no currents in oocytes injected with the cerebellar poly(A)(+) RNA alone, the poly(A)(+) RNA and cRNA for GIRK1 or GIRK2, or only cRNAs for GIRK1 and GIRK2. These findings indicate that GABA-B receptors in the rat cerebellum were functionally expressed in Xenopus oocytes and activated the cloned GIRKs composed of GIRK1 and GIRK2 as heteromultimers.
引用
收藏
页码:583 / 587
页数:5
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