ACTIVATION OF INWARDLY RECTIFYING POTASSIUM CHANNELS (GIRK1) BY COEXPRESSED RAT-BRAIN CANNABINOID RECEPTORS IN XENOPUS OOCYTES

被引：127

作者：

HENRY, DJ

CHAVKIN, C

机构：

[1] UNIV WASHINGTON,DEPT PHARMACOL,SJ-30,SEATTLE,WA 98195

[2] CALTECH,DIV ENGN & APPL SCI,PASADENA,CA 91125

来源：

NEUROSCIENCE LETTERS | 1995年 / 186卷 / 2-3期

关键词：

K+ CHANNEL; MARIJUANA; ADDICTION; CANNABIS; G-PROTEIN COUPLED RECEPTOR; KGA;

D O I：

10.1016/0304-3940(95)11289-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The neuronal cannabinoid receptor clone was expressed in Xenopus laevis oocytes as shown by the expression of saturable [H-3]WIN 55,212-2 binding sites. Go-expression of the cannabinoid receptor with cRNA coding for the G-protein-gated inwardly rectifying K+ channel (GIRK1) resulted in oocytes exhibiting large inward K+ currents in response to the cannabinoid agonist WIN 55,212-2. The activation of the potassium current by WIN 55,212-2 was dose-dependent with an EC(50) Of 630 nM. These results suggest that activation of inwardly rectifying K+ channels may be an additional effector mechanism for brain cannabinoid receptors.

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页码：91 / 94

页数：4

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