High-resolution genotyping of Chlamydia trachomatis from recurrent urogenital infections

被引：27

作者：

Pedersen, LN

Kjær, HO

Moller, JK

Orntoft, TF

Ostergaard, L

机构：

[1] Aarhus Kommune Hosp, Dept Clin Microbiol, DK-8000 Aarhus, Denmark

[2] Marselisborg Hosp, Dept Infect Dis, DK-8000 Aarhus, Denmark

[3] Horsens Cty Hosp, Dept Internal Med, DK-8700 Horsens, Denmark

[4] Skejby Hosp, Dept Clin Biochem, DK-8200 Aarhus N, Denmark

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 08期

关键词：

D O I：

10.1128/JCM.38.8.3068-3071.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A method for Chlamydia trachomatis restriction fragment length polymorphism (RFLP) analysis and complete sequencing of omp1 was developed for use on samples collected at home, and results were compared. Genotyping by sequencing was superior to RFLP analysis. The omp1 gene in 31 clinical strains harbored few mutations compared to the same gene in ATCC reference strains. Follow-up samples obtained during a 24-week period from 31 patients showed recurrence with the same genotype in five cases and a new genotype in one case.

引用

收藏

页码：3068 / 3071

页数：4

相关论文

共 16 条

[1] Home sampling versus conventional contact tracing for detecting Chlamydia trachomatis infection in male partners of infected women:: randomised study [J].

Andersen, B ;

Ostergaard, L ;

Moller, JK ;

Olesen, F .

BMJ-BRITISH MEDICAL JOURNAL, 1998, 316 (7128) :350-351

[2]

Borrego MJ, 1997, GENITOURIN MED, V73, P561

[3] GENITAL CHLAMYDIAL INFECTIONS - EPIDEMIOLOGY AND REPRODUCTIVE SEQUELAE [J].

CATES, W ;

WASSERHEIT, JN .

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1991, 164 (06) :1771-1781

[4]

ELEY A, 1993, GENITOURIN MED, V69, P481

[5]

Kjær HO, 2000, SEX TRANSM INFECT, V76, P169, DOI 10.1136/sti.76.3.169

[6] IMPROVED PCR SENSITIVITY FOR DIRECT GENOTYPING OF CHLAMYDIA-TRACHOMATIS SEROVARS BY USING A NESTED PCR [J].

LAN, J ;

OSSEWAARDE, JM ;

WALBOOMERS, JMM ;

MEIJER, CJLM ;

VANDENBRULE, AJC .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (02) :528-530

[7] DIRECT DETECTION AND GENOTYPING OF CHLAMYDIA-TRACHOMATIS IN CERVICAL SCRAPES BY USING POLYMERASE CHAIN-REACTION AND RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM ANALYSIS [J].

LAN, J ;

WALBOOMERS, JMM ;

ROOSENDAAL, R ;

VANDOORNUM, GJJ ;

MACLAREN, DM ;

MEIJER, CJLM ;

VANDENBRULE, AJC .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (05) :1060-1065

[8] COMPARISON OF PLASMID-BASED AND CHROMOSOME-BASED POLYMERASE CHAIN-REACTION ASSAYS FOR DETECTING CHLAMYDIA-TRACHOMATIS NUCLEIC-ACIDS [J].

MAHONY, JB ;

LUINSTRA, KE ;

SELLORS, JW ;

CHERNESKY, MA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (07) :1753-1758

[9] Impact of menstrual cycle on the diagnostic performance of LCR, TMA, and PCE for detection of Chlamydia trachomatis in home obtained and mailed vaginal flush and urine samples [J].

Moller, JK ;

Andersen, B ;

Olesen, F ;

Lignell, T ;

Ostergaard, L .

SEXUALLY TRANSMITTED INFECTIONS, 1999, 75 (04) :228-230

[10]

Morré SA, 1998, J CLIN MICROBIOL, V36, P345