Ratiometric Two-Photon Fluorescent Probe for Quantitative Detection of β-Galactosidase Activity in Senescent Cells

被引：137

作者：

Lee, Hyo Won ^{[1
]}

Heo, Cheol Ho ^{[1
]}

Sen, Debabrata ^{[1
]}

Byun, Hae-Ok ^{[2
,3
]}

Kwak, In Hae ^{[2
,3
]}

Yoon, Gyesoon ^{[2
,3
]}

Kim, Hwan M-Yung ^{[1
]}

机构：

[1] Ajou Univ, Dept Energy Syst Res, Suwon 443749, Gyeonggi Do, South Korea

[2] Ajou Univ, Sch Med, Dept Biochem, Suwon 443721, Gyeonggi Do, South Korea

[3] Ajou Univ, Sch Med, Dept Biomed Sci, Suwon 443721, Gyeonggi Do, South Korea

来源：

ANALYTICAL CHEMISTRY | 2014年 / 86卷 / 20期

基金：

新加坡国家研究基金会;

关键词：

FIBROBLAST HS68 CELLS; IN-VIVO; LIVE TISSUES; METAL-IONS; GENE; BIOMARKER; SCAFFOLD; CULTURE;

D O I：

10.1021/ac5031013

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We reported a ratiometric two-photon fluorescent probe (SG1) for b-galactosidase (b-gal) and its application to quantitative detection of b-gal activity during cellular senescence in live cells and in aged tissues. This probe is characterized by a significant two-photon excited fluorescence, a marked blue-to-yellow emission color change in response to b-gal, easy loading, insensitivity to pH and reactive oxygen species (ROS), high photostability, and low cytotoxicity. In addition, we show that SG1 labeling is an effective tool for quantitative detection of senescence-associated b-gal activity at the subcellular level in situ. This finding demonstrates that SG1 will find useful applications in biomedical research, including studies of cell aging.

引用

页码：10001 / 10005

页数：5

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