Immobilization of antibodies on alginate-chitosan beads

被引：74

作者：

Albarghouthi, M

Abu Fara, D

Saleem, M

El-Thaher, T

Matalka, K

Badwan, A

机构：

[1] Jordanian Pharmaceut Mfg Co, Naor 11710, Jordan

[2] Univ Jordan, Dept Chem Engn, Amman 11942, Jordan

[3] Univ Petra, Fac Pharm & Med Technol, Amman, Jordan

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2000年 / 206卷 / 1-2期

关键词：

immobilization; antibodies; alginate-chitosan; beads; anti-lisinopril IgG;

D O I：

10.1016/S0378-5173(00)00470-1

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

An anti-hapten IgG was covalently immobilized on glutaraldehyde-activated alginate-chitosan gel beads. The antibody immobilization efficiency was influenced by glutaraldehyde-bead reaction time, IgG concentration and pH. In addition, immobilization conditions such as glutaraldehyde, and antibody concentrations influenced antibody hapten binding affinity. The immobilized IgG on the beads was stable and no reduction in the percent binding to hapten was noticed following 25 days of storage. It was concluded that antibodies could be successfully immobilized on alginate-chitosan gel beads. Such a system can be applied for the development of immunoaffinity purification and immunoassays. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：23 / 34

页数：12

共 23 条

[1]

[Anonymous], 1988, Antibodies: A Laboratory Manual

[2] Protein loss by the microencapsulation of an enzyme (lactase) in alginate beads [J].