Self-assembled biomimetic monolayers using phospholipid-containing disulfides

被引:64
作者
Chung, YC
Chiu, YH
Wu, YW
Tao, YT
机构
[1] Natl Univ Kaohsiung, Dept Chem & Mat Engn, Kaohsiung 811, Taiwan
[2] I Shou Univ, Dept Biomed Engn, Kaohsiung 840, Taiwan
[3] I Shou Univ, Inst Biotechnol & Chem Engn, Kaohsiung 840, Taiwan
[4] Acad Sinica, Inst Chem, Taipei 11529, Taiwan
关键词
self-assembled monolayer; disulfides; biocompatibility; phosphorylcholine;
D O I
10.1016/j.biomaterials.2004.06.043
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Several phospholipid-based disulfide molecules were synthesized and attached onto the gold-coated silicon wafer using the self assembling method. The syntheses of these surface-modifying agents were conducted by introducing bromoethylphosphorate (PBr), phosphorylcholine (PC) or phosphorylethanolamine (PE) groups on the terminals of a dialkyl disulfide. After disulfides adsorption Into gold substrate surfaces, the composition, the film thickness, and the conformational order of self-assembled monolayer surfaces were explored and discussed in detail based on reflection-absorption infrared spectroscopy, contact angle measurement, Auger electron spectroscopy, X-ray photoelectron spectroscopy, and so on. The monolayer having the PBr end group could also be converted to a PC surface by treating with trimethylamine. The model functional surfaces of Au-SC1l-PC, -PE, -PBr, -OH or corresponding mixed layers were used to mimic biomembrane surfaces. The monolayer having PC groups was found to reduce fibrinogen adsorption as evaluated from protein adsorption experiments using quartz crystal microbalance. It also showed relatively low platelet adherence compare to the glass, PBr and PE surfaces. The cell viability test also revealed that the PC surface displayed lower cytotoxicity than other surfaces. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:2313 / 2324
页数:12
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