Parameters associated with optimum two-step freezing of rumen ciliate Entodinium caudatum

被引:7
作者
Kisidayova, S
机构
[1] Slovak Academy of Sciences, Inst. Anim. Physiol., 040 01 Kosice
关键词
cryopreservation; Entodinium caudatum; recultivation; rumen ciliate protozoan;
D O I
10.1016/S0167-7012(97)00053-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The monofaunated rumen ciliate Entodinium caudatum used in the optimum two-step freezing protocol shows a high cell survival. The decisive components of the recultivation were the inoculum size (>2000 ml(-1)) and the feed (wheat flour plus ground dried white clover) and to a lesser extent, the composition of the tested culture media. The addition of glucose to the thawed cell suspension stabilized the development of protozoal culture during recultivation. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:119 / 124
页数:6
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