Autofluorescence and mineral content of carious dentine: Scanning optical and backscattered electron microscopic studies

被引:47
作者
Banerjee, A [1 ]
Boyde, A [1 ]
机构
[1] UCL, Dept Anat & Dev Biol, London, England
基金
英国惠康基金;
关键词
backscattered scanning electron microscopy; confocal microscopy; dentine caries; fluorescence;
D O I
10.1159/000016456
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
The aim of this study was to investigate the extent of natural intrinsic fluorescence in carious human dentine and any correspondence of such autofluorescence (AF) to the mineral distribution within the lesion. Two investigative techniques were used, both employing the same sample set and fields in diamond-polished block surfaces of polymethylmethacrylate-embedded carious teeth. AF at emission wavelengths >515 nm, excited by 488-nm laser light, was assessed using a confocal laser scanning optical microscope (CLSM), the recordings made under standard operating conditions. The relative mineral content was assessed using digital backscattered scanning electron microscopy (20 kV BSE-SEM). The AF intensity correlated with the level of demineralisation as seen by BSE, but the depth of the lesion as seen by CLSM was significantly greater implying that the AF is not directly related to the mineral component.
引用
收藏
页码:219 / 226
页数:8
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