Quantitation of Cremophor EL in human plasma samples using a colorimetric dye-binding microassay

被引:52
作者
Sparreboom, A
Loos, WJ
Verweij, J
de Vos, AI
van der Burg, MEL
Stoter, G
Nooter, K
机构
[1] Rotterdam Canc Inst, Daniel den Hoed Klin, Dept Med Oncol, Lab Expt Chemotherapy & Pharmacol, NL-3008 AE Rotterdam, Netherlands
[2] Univ Rotterdam Hosp, NL-3008 AE Rotterdam, Netherlands
关键词
D O I
10.1006/abio.1997.2467
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This paper describes an analytical procedure for the quantitative determination of the pharmaceutical vehicle Cremophor EL in human plasma samples. The procedure is based on rapid binding of Coomassie brilliant blue G-250 to Cremophor EL following plasma protein precipitation with acetonitrile and analyte extraction with n-butylchloride. The binding of the dye to Cremophor EL causes a shift in the absorption maximum from 465 to 624 nm, which is monitored using an automated microplate-absorbance reader. The assay permits estimation of Cremophor EL concentrations in the range 0.05-1.00% (v/v) in 50 mu L of human plasma, with percentage deviation and precision of less than or equal to 12 and less than or equal to 15%, respectively. The assay was subsequently used to measure Cremophor EL concentrations in plasma samples in support of a project to develop a pharmacokinetic model for this compound in patients receiving paclitaxel. (C) 1998 Academic Press.
引用
收藏
页码:171 / 175
页数:5
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