A protein-driven DNA device that measures the excess binding energy of proteins that distort DNA

被引：49

作者：

Shen, WQ

Bruist, MF

Goodman, SD

Seeman, NC ^{[1
]}

机构：

[1] NYU, Dept Chem, New York, NY 10003 USA

[2] Univ Sci Philadelphia, Dept Chem, Philadelphia, PA 19104 USA

[3] Univ So Calif, Dept Mol & Computat Biol, Los Angeles, CA 90089 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 36期

关键词：

DNA structures; fluorescent probes; molecular devices; proteins;

D O I：

10.1002/anie.200460302

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A DNA nanomechanical device was constructed by linking two triple-helical DNA motifs with a shaft of conventional DNA bearing the binding site for the integration host factor (IHF, shown as green ellipse in the picture). Binding of IHF to this site causes marked shaft bending, which can be monitored by fluorescence resonance energy transfer between donor (D) and acceptor (A) dyes.

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收藏

页码：4750 / 4752

页数：3

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