Vagaries of conditional gene targeting

被引：335

作者：

Schmidt-Supprian, Marc ^{[1
]}

Rajewsky, Klaus

机构：

[1] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA

[2] CBR, Inst Biomed Res, Boston, MA 02115 USA

来源：

NATURE IMMUNOLOGY | 2007年 / 8卷 / 07期

关键词：

D O I：

10.1038/ni0707-665

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Conditional gene targeting based on excision or inversion of loxP-flanked DNA segments by Cre recombinase is a powerful technology for the analysis of gene function, but unexpected expression patterns of cre transgenes, variability of recombination efficiency depending on the target gene and potential toxicity of Cre recombinase represent serious challenges for the experimenter.

引用

页码：665 / 668

页数：4

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