Distribution of IRAG and cGKI-isoforms in murine tissues

被引：77

作者：

Geiselhöringer, A ^{[1
]}

Gaisa, A ^{[1
]}

Hofmann, F ^{[1
]}

Schlossmann, J ^{[1
]}

机构：

[1] Tech Univ Munich, Inst Pharmakol & Toxikol, D-80802 Munich, Germany

来源：

FEBS LETTERS | 2004年 / 575卷 / 1-3期

关键词：

tissue distribution; immunohistochemistry; cGMP-dependent protein kinase; in-situ hybridization; IRAG; smooth muscle;

D O I：

10.1016/j.febslet.2004.08.030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

cGMP kinase I (cGKI) signaling modulates multiple physiological processes including smooth muscle relaxation. The expression of cGKI and its substrate IRAG (Inositol 1,4,5-trisphosphate receptor associated cGMP kinase substrate) was studied. IRAG and cGKI were colocalized in the smooth muscle of aorta and colon. IRAG was present in the thalamus and in most of the myenteric plexus in the absence of cGKI. Coexpression of IRAG and cGKIbeta or cGKIalpha in COS-7 cells revealed that IRAG recruits cGKIbeta but not cGKIalpha to the endoplasmic reticulum. These results suggest that IRAG may be involved in cGKI-dependent and -independent pathways. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：19 / 22

页数：4

共 18 条

[11] Differential distribution of four hyperpolarization-activated cation channels in mouse brain [J].

Moosmang, S ;

Biel, M ;

Hofmann, F ;

Ludwig, A .

BIOLOGICAL CHEMISTRY, 1999, 380 (7-8) :975-980

[12]

Pfeifer A, 1999, Rev Physiol Biochem Pharmacol, V135, P105, DOI 10.1007/BFb0033671

[13] Defective smooth muscle regulation in cGMP kinase I-deficient mice [J].

Pfeifer, A ;

Klatt, P ;

Massberg, S ;

Ny, L ;

Sausbier, M ;

Hirneiss, C ;

Wang, GX ;

Korth, M ;

Aszódi, A ;

Andersson, KE ;

Krombach, F ;

Mayerhofer, A ;

Ruth, P ;

Fässler, R ;

Hofmann, F .

EMBO JOURNAL, 1998, 17 (11) :3045-3051

[14] TRANSFECTED CGMP-DEPENDENT PROTEIN-KINASE SUPPRESSES CALCIUM TRANSIENTS BY INHIBITION OF INOSITOL 1,4,5-TRISPHOSPHATE PRODUCTION [J].

RUTH, P ;

WANG, GX ;

BOEKHOFF, I ;

MAY, B ;

PFEIFER, A ;

PENNER, R ;

KORTH, M ;

BREER, H ;

HOFMANN, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (07) :2623-2627

[15] Mechanisms of NO/cGMP-dependent vasorelaxation [J].

Sausbier, M ;

Schubert, R ;

Voigt, V ;

Hirneiss, C ;

Pfeifer, A ;

Korth, M ;

Kleppisch, T ;

Ruth, P ;

Hofmann, F .

CIRCULATION RESEARCH, 2000, 87 (09) :825-830

[16] Regulation of intracellular calcium by a signalling complex of IRAG, IP3 receptor and cGMP kinase Iβ [J].

Schlossmann, J ;

Ammendola, A ;

Ashman, K ;

Zong, XG ;

Huber, A ;

Neubauer, G ;

Wang, GX ;

Allescher, HD ;

Korth, M ;

Wilm, M ;

Hofmann, F ;

Ruth, P .

NATURE, 2000, 404 (6774) :197-201

[17] SIGNAL-TRANSDUCTION AND REGULATION IN SMOOTH-MUSCLE [J].

SOMLYO, AP ;

SOMLYO, AV .

NATURE, 1994, 372 (6503) :231-236

[18] Regulation of myosin phosphatase by a specific interaction with cGMP-dependent protein kinase Iα [J].

Surks, HK ;

Mochizuki, N ;

Kasai, Y ;

Georgescu, SP ;

Tang, KM ;

Ito, M ;

Lincoln, TM ;

Mendelsohn, ME .

SCIENCE, 1999, 286 (5444) :1583-1587

← 1 2 →