Pathways involved in gut mucosal barrier dysfunction induced in adult rats by maternal deprivation: corticotrophin-releasing factor and nerve growth factor interplay

被引:115
作者
Barreau, Frederick
Cartier, Christel
Leveque, Mathilde
Ferrier, Laurent
Moriez, Raphael
Laroute, Valerie
Rosztoczy, Andras
Fioramonti, Jean
Bueno, Lionel
机构
[1] INRA, Neurogastroenterol & Nutr Unit, F-31931 Toulouse 9, France
[2] Ecole Natl Vet Toulouse, Physiopathol & Toxicol Unit, Toulouse, France
[3] Univ Szeged, Dept Internal Med, H-6720 Szeged, Hungary
来源
JOURNAL OF PHYSIOLOGY-LONDON | 2007年 / 580卷 / 01期
关键词
D O I
10.1113/jphysiol.2006.120907
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Neonatal maternal deprivation (NMD) increases gut paracellular permeability (GPP) through mast cells and nerve growth factor (NGF), and modifies corticotrophin-releasing factor (CRF) and corticosterone levels. CRF, corticosterone and mast cells are involved in stress-induced mucosal barrier impairment. Consequently, this study aimed to specify whether corticosteronaemia and colonic expression of both preproCRF and CRF are modified by NMD, and to determine if altered expression may participate in the elevated GPP in connection with NGF and mast cells. Male Wistar rat pups were either separated from postnatal days 2-14, or left undisturbed with their dam. At 12 weeks of age, adult rats were treated with mifepristone (an antagonist of corticoid receptors), alpha-helical CRF(9-41) (a non-specific CRF receptor antagonist), or SSR-125543 (CRF-R-1 receptor antagonist). We also determined corticosteronaemia and both colonic preproCRF and CRF expression. Then, control rats were treated by CRF, doxantrazole (mast cell stabilizer), BRX-537A (a mast cell activator) and anti-NGF antibody. NMD did not modify colonic CRF level but increased colonic preproCRF expression and corticosteronaemia. Peripheral CRF, via CRF-R-1 receptor, but not corticosterone, was involved in the elevated GPP observed in these rats, through a mast-cell-mediated mechanism, since the increase of GPP induced by exogenous CRF was abolished by doxantrazole. Anti-NGF antibody treatment also reduced the elevated GPP induced by CRF or BRX-537A. CRF acts through CRF-R-1 receptors to stimulate NGF release from mast cells, which participates in the elevated GPP observed in NMD adult rats. This suggests that early traumatic experience induced neuro-endocrine dysfunction, involved in alterations of gut mucosal barrier.
引用
收藏
页码:347 / 356
页数:10
相关论文
共 52 条
[31]   Stress and exacerbation in ulcerative colitis:: A prospective study of patients enrolled in remission [J].
Levenstein, S ;
Prantera, C ;
Varvo, V ;
Scribano, ML ;
Andreoli, A ;
Luzi, C ;
Arcà, M ;
Berto, E ;
Milite, G ;
Marcheggiano, A .
AMERICAN JOURNAL OF GASTROENTEROLOGY, 2000, 95 (05) :1213-1220
[32]   Primary social relationships influence the development of the hypothalamic-pituitary-adrenal axis in the rat [J].
Levine, S .
PHYSIOLOGY & BEHAVIOR, 2001, 73 (03) :255-260
[33]   RECOLLECTION OF CHILDHOOD EVENTS IN ADULTS WITH IRRITABLE-BOWEL-SYNDROME [J].
LOWMAN, BC ;
DROSSMAN, DA ;
CRAMER, EM ;
MCKEE, DC .
JOURNAL OF CLINICAL GASTROENTEROLOGY, 1987, 9 (03) :324-330
[34]  
MAYER EA, 2001, GASTROINTESTINAL LIV, V280, pG519
[35]   Environmental stress-induced gastrointestinal permeability is mediated by endogenous glucocorticoids in the rat [J].
Meddings, JB ;
Swain, MG .
GASTROENTEROLOGY, 2000, 119 (04) :1019-1028
[36]   PERIODIC MATERNAL-DEPRIVATION ALTERS STRESS-RESPONSE IN ADULT OFFSPRING - POTENTIATES THE NEGATIVE FEEDBACK-REGULATION OF RESTRAINT STRESS-INDUCED ADRENOCORTICAL-RESPONSE AND REDUCES THE FREQUENCIES OF OPEN FIELD-INDUCED BEHAVIORS [J].
OGAWA, T ;
MIKUNI, M ;
KURODA, Y ;
MUNEOKA, K ;
MORI, KJ ;
TAKAHASHI, K .
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR, 1994, 49 (04) :961-967
[37]   STIMULATION OF THE PITUITARY-ADRENOCORTICAL AXIS AND INDUCTION OF TYROSINE-HYDROXYLASE BY NERVE GROWTH-FACTOR ARE NOT DEPENDENT ON MOUSE SUB-MAXILLARY GLAND ISORENIN [J].
OTTEN, U ;
GOEDERT, M ;
BAUMANN, JB ;
GIRARD, J .
BRAIN RESEARCH, 1981, 217 (01) :207-211
[38]   STIMULATION OF THE PITUITARY-ADRENOCORTICAL AXIS BY NERVE GROWTH-FACTOR [J].
OTTEN, U ;
BAUMANN, JB ;
GIRARD, J .
NATURE, 1979, 282 (5737) :413-414
[39]   ROLE OF MAST-CELLS IN ION-TRANSPORT ABNORMALITIES ASSOCIATED WITH INTESTINAL ANAPHYLAXIS - CORRECTION OF THE DIMINISHED SECRETORY RESPONSE IN GENETICALLY MAST CELL-DEFICIENT W/WV MICE BY BONE-MARROW TRANSPLANTATION [J].
PERDUE, MH ;
MASSON, S ;
WERSHIL, BK ;
GALLI, SJ .
JOURNAL OF CLINICAL INVESTIGATION, 1991, 87 (02) :687-693
[40]   Psychosocial aspects of Crohn's disease [J].
Ringel, Y ;
Drossman, DA .
SURGICAL CLINICS OF NORTH AMERICA, 2001, 81 (01) :231-+