Fission yeast Slp1: An effector of the Mad2-dependent spindle checkpoint

被引：314

作者：

Kim, SH

Lin, DP

Matsumoto, S

Kitazono, A

Matsumoto, T ^{[1
]}

机构：

[1] Albert Einstein Coll Med, Dept Radiat Oncol, Bronx, NY 10461 USA

[2] Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA

来源：

SCIENCE | 1998年 / 279卷 / 5353期

关键词：

D O I：

10.1126/science.279.5353.1045

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mad2 is a component of the spindle checkpoint, which delays the onset of anaphase until all chromosomes are attached to the spindle. Mad2 formed a complex with Slp1, a WD (tryptophan-aspartic acid)-repeat protein essential for the onset of anaphase. When the physical interaction between the two proteins was disrupted, the spindle checkpoint was no longer functional. Post-anaphase events such as chromosome decondensation and the next round of DNA replication were not delayed even when the spindle assembly was incomplete. This relief of dependence appears to be a result of deregulation of ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex.

引用

页码：1045 / 1047

页数：3

共 31 条

[1] Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores [J].