Structure of in-serum transfecting DNA-cationic lipid complexes

被引：71

作者：

Boukhnikachvili, T

AguerreChariol, O

Airiau, M

Lesieur, S

Ollivon, M

Vacus, J

机构：

[1] RHONE POULENC RORER,CTR RECH VITRY ALFORTVILLE,F-94400 VITRY SUR SEINE,FRANCE

[2] RHONE POULENC RORER GENCELL,CTR RECH VITRY ALFORTVILLE,F-94400 VITRY SUR SEINE,FRANCE

[3] RHONE POULENC,F-93308 AUBERVILLIERS,FRANCE

[4] FAC PHARM PARIS,CNRS,URA 1218,F-92296 CHATENAY MALABR,FRANCE

来源：

FEBS LETTERS | 1997年 / 409卷 / 02期

关键词：

transfection efficiency; cationic lipid; DNA condensation; cryo-transmission electron microscopy; X-ray diffraction; lamellar structure;

D O I：

10.1016/S0014-5793(97)00505-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Noticeable modifications of in-serum transfection efficiency of dioctadecylamidoglycyl-spermine (DOGS)-DNA complexes are observed, depending on DNA condensation conditions. The structures of the complexes are studied, keeping in mind the variability of lipid polymorphism, by cryo-transmission electron microscopy and X-ray diffraction. By increasing both pH and ionic strength, well-organised lamellar structures with a period of 65 Angstrom replace supramicellar aggregates. A relationship between the structures and their in-vitro transfection activity is established. Efficiency in the presence of serum is maintained when a lamellar arrangement is involved. (C) 1997 Federation of European Biochemical Societies.

引用

页码：188 / 194

页数：7

共 41 条

[1] Cryotransmission electron microscopy of thin vitrified samples [J].