Simple method for the quantitative examination of extra column band broadening in microchromatographic systems

被引：13

作者：

Beisler, AT ^{[1
]}

Schaefer, KE ^{[1
]}

Weber, SG ^{[1
]}

机构：

[1] Univ Pittsburgh, Dept Chem, Pittsburgh, PA 15260 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 986卷 / 02期

关键词：

band broadening; post-column reactors;

D O I：

10.1016/S0021-9673(02)02018-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In recent years capillary chromatography has gained popularity for trace analyses. Most often UV or electrochemical detection is employed because the small peak volumes make post-column derivatization challenging. We have developed a simple method based on flow injection for determining contributions to peak broadening from post-column reactors. The only requirement for application of our methodology is that diffusion be in the Taylor regime so that radial concentration gradients are relaxed enabling mixing purely by diffusion. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：247 / 251

页数：5

共 14 条

[1]

BETTERIDGE D, 1978, ANAL CHEM, V50, pA832, DOI 10.1021/ac50031a001

[2] Trace-level amino acid analysis by capillary liquid chromatography and application to in vivo microdialysis sampling with 10-s temporal resolution [J].