A three-step PCR protocol for construction of chimeric proteins

被引：19

作者：

Grandori, R ^{[1
]}

Struck, K ^{[1
]}

Giovanielli, K ^{[1
]}

Carey, J ^{[1
]}

机构：

[1] Princeton Univ, Dept Chem, Princeton, NJ 08544 USA

来源：

PROTEIN ENGINEERING | 1997年 / 10卷 / 09期

关键词：

chimeric proteins; PCR; protein structure; protein subdomains; WrbA;

D O I：

10.1093/protein/10.9.1099

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A general method is described for creating chimeric proteins by transposition of subdomain-sized gene fragments. The method uses three sequential PCR steps to circumvent several limitations inherent in transposition of small insertions and has been optimized to avoid purification of intermediate products. The sequence-independence of the method permits flexibility in the choice of host molecule and insertion site.

引用

页码：1099 / 1100

页数：2