Microencapsulated bacterial cells can be used to produce the enzyme feruloyl esterase: preparation and in-vitro analysis

被引：23

作者：

Bhathena, Jasmine

Kulamarva, Arun

Urbanska, Aleksandra Malgorzata

Martoni, Christopher

Prakash, Satya

机构：

[1] McGill Univ, Fac Med, Dept Biomed Engn, Biomed Technol & Cell Therapy Res Lab, Montreal, PQ H3A 2B4, Canada

[2] McGill Univ, Fac Med, Artificial Cells & Organs Res Ctr, Montreal, PQ H3A 2B4, Canada

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2007年 / 75卷 / 05期

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

D O I：

10.1007/s00253-007-0908-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Biotechnological production of ferulic acid, a precursor of vanillin, is an attractive alternative for various industries due to the high price and demand for natural ferulic acid. Feruloyl esterase has been identified as a key enzyme involved in microbial transformations of ferulic acid to vanillin. Several fungal feruloyl esterases have been purified and characterized for their use in the production of ferulic acid. This paper, for the first time, discusses the use of lactic acid bacteria for the production of ferulic acid. Specifically, we have used Lactobacillus cells and microencapsulation so that ferulic acid can be produced continuously using various types of fermentation systems. Bacteria were encapsulated in alginate-poly-L-lysine-alginate (APA) microcapsules, and the production of ferulic acid by lactobacilli was detected using a real-time high-performance liquid chromatography (HPLC)-based assay. Results show that ferulic acid can be produced using microencapsulated Lactobacillus fermentum (ATCC 11976) with significant levels of biological feruloyl esterase activity.

引用

页码：1023 / 1029

页数：7

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