Smart polymeric carriers for enhanced intracellular delivery of therapeutic macromolecules

被引：59

作者：

El-Sayed, MEH ^{[1
]}

Hoffman, AS ^{[1
]}

Stayton, PS ^{[1
]}

机构：

[1] Univ Washington, Dept Bioengn, Seattle, WA 98195 USA

来源：

EXPERT OPINION ON BIOLOGICAL THERAPY | 2005年 / 5卷 / 01期

关键词：

antisense delivery; encrypted polymers; gene detivery; intracellular delivery; PDSA polymers; polymer therapeutics; poly(propylacrylic acid) polymers; smart polymers; vaccine delivery;

D O I：

10.1517/14712598.5.1.23

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Limited cytoplasmic delivery of enzyme-susceptible drugs remains a significant challenge facing the development of protein and nucleic acid therapies that act in intracellular compartments. Researchers have examined several approaches, including fusogenic proteins and protein transduction domains, to enhance the intracellular delivery of the therapeutic cargo. This review summarises efforts to develop 'smart' pH-sensitive and membrane-destabilising polymers that can shuttle therapeutic peptide, protein and nucleic acid molecules past the endosomal membrane into the cytoplasm of targeted cells. Several classes of 'smart' non-degradable polymeric carriers have been developed that have proved effective both in vitro and in vivo in enhancing the cytoplasmic delivery of a variety of therapeutic molecules.

引用

页码：23 / 32

页数：10

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